AB201255

Alexa Fluor® 488 Anti-ENT2 抗体 [EPR11674]

Alexa Fluor® 488 Anti-ENT2 antibody [EPR11674]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal ENT2 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

別名を表示する

DER12, ENT2, HNP36, SLC29A2, Equilibrative nucleoside transporter 2, hENT2, 36 kDa nucleolar protein HNP36, Delayed-early response protein 12, Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter, Solute carrier family 29 member 2, Equilibrative NBMPR-insensitive nucleoside transporter

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ENT2 antibody [EPR11674] (AB201255)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ENT2 antibody [EPR11674] (AB201255)

ab201255 staining ENT2 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201255 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR11674

アイソタイプ

IgG

標識

Alexa Fluor® 488

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

交差種

Human

アプリケーション

ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ENT2 also known as Equilibrative Nucleoside Transporter 2 facilitates the transport of nucleosides across cellular membranes. It weighs approximately 56 kDa. The transporter is widely expressed in various tissues including the brain heart and placenta. By allowing the movement of nucleosides ENT2 plays a fundamental role in nucleoside salvage an important process for maintaining cellular nucleotide pools especially in energy-demanding tissues.

Biological function summary

ENT2 participates in the regulation of intracellular nucleoside levels which impacts DNA and RNA synthesis. It operates individually and does not form part of a larger protein complex allowing it to specifically affect the transport rates based on cellular requirements. This role supports essential cellular processes like cell growth and division highlighting ENT2 as an important regulator in genomic stability and cellular homeostasis.

Pathways

ENT2 plays major roles in the purine and pyrimidine salvage pathways. These pathways involve essential enzymes that facilitate the recycling of nucleosides for nucleotide synthesis. Related proteins include ENT1 another member of the equilibrative nucleoside transporter family which shares functional similarities in nucleoside transport across membranes and influences the dynamics of these pathways.

Dysfunction of ENT2 links to conditions like myocardial ischemia and neurodegenerative disorders. In these conditions ENT2's role in regulating nucleoside availability directly impacts cellular stress responses. For instance its interaction with nucleoside transport can modulate the extracellular adenosine levels influencing the purinergic signaling pathways. In cardiac tissues altered ENT2 function affects the availability of nucleosides during ischemic events potentially exacerbating tissue damage while in neurodegenerative contexts its regulation of nucleoside levels can impact neuron survival and function potentially involving proteins like adenosine deaminase.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Bidirectional uniporter involved in the facilitative transport of nucleosides and nucleobases, and contributes to maintaining their cellular homeostasis (PubMed : 10722669, PubMed : 12527552, PubMed : 12590919, PubMed : 16214850, PubMed : 21795683, PubMed : 9396714, PubMed : 9478986). Functions as a Na(+)-independent, passive transporter (PubMed : 9478986). Involved in the transport of nucleosides such as inosine, adenosine, uridine, thymidine, cytidine and guanosine (PubMed : 10722669, PubMed : 12527552, PubMed : 12590919, PubMed : 16214850, PubMed : 21795683, PubMed : 9396714, PubMed : 9478986). Also able to transport purine nucleobases (hypoxanthine, adenine, guanine) and pyrimidine nucleobases (thymine, uracil) (PubMed : 16214850, PubMed : 21795683). Involved in nucleoside transport at basolateral membrane of kidney cells, allowing liver absorption of nucleoside metabolites (PubMed : 12527552). Mediates apical nucleoside uptake into Sertoli cells, thereby regulating the transport of nucleosides in testis across the blood-testis-barrier (PubMed : 23639800). Mediates both the influx and efflux of hypoxanthine in skeletal muscle microvascular endothelial cells to control the amount of intracellular hypoxanthine available for xanthine oxidase-mediated ROS production (By similarity).. Isoform 3. Non functional nucleoside transporter protein for adenosine or thymidine transport. Does not express on cell membrane.

See full target information SLC29A2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:480 PubMed40164758

2025

Protein covariation networks for elucidating ferroptosis inducer mechanisms and potential synergistic drug targets.

Applications

Unspecified application

Species

Unspecified reactive species

Rina Kunishige,Yoshiyuki Noguchi,Naomi Okamoto,Lei Li,Akito Ono,Masayuki Murata,Fumi Kano

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-ENT2 抗体 [EPR11674]

Alexa Fluor® 488 Anti-ENT2 antibody [EPR11674]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ENT2 antibody [EPR11674] (AB201255)

関連する標識済み抗体及び組成の異なる製品 (8)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise