JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317596

Alexa Fluor® 488 Anti-Dysferlin 抗体 [JAI-1-49-3]

Alexa Fluor® 488 Anti-Dysferlin antibody [JAI-1-49-3]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Dysferlin antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-Fr, ICC/IF, IHC-P and reacts with Mouse, Human samples.

別名を表示する

FER1L1, DYSF, Dysferlin, Dystrophy-associated fer-1-like protein, Fer-1-like protein 1

1 Images
Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Dysferlin antibody [JAI-1-49-3] (AB317596)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Dysferlin antibody [JAI-1-49-3] (AB317596)

Immunofluorescence staining of Dysferlin in a section of frozen mouse normal skeletal muscle.

The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BOND™. The section was incubated at room temperature for 1 hour with ab317596 at 1/50 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

関連する標識済み抗体及び組成の異なる製品 (7)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

JAI-1-49-3

アイソタイプ

IgG

標識

Alexa Fluor® 488

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

No

交差種

Mouse, Human, Mouse

アプリケーション

ICC/IF, IHC-P, IHC-Fr

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Dysferlin also known by its alternate names DYSF and CAPN3 is a protein characterized by a mass of approximately 237 kilodaltons. It belongs to the ferlin family and is mainly expressed in skeletal and cardiac muscle tissues. This protein contains multiple C2 domains which are significant for its membrane-trafficking roles. Dysferlin has an important role at the muscle surface particularly in the repair of sarcolemma breaches which helps maintain muscle cell integrity.
Biological function summary

The action of dysferlin impacts several molecular processes critical for muscle function and maintenance often interacting as part of a larger protein complex. It facilitates membrane repair by promoting vesicle fusion events and recruits other proteins to damaged sites. Such action is vital in restoring cellular structure after membrane damage particularly under the mechanical stress experienced in muscle tissues.

Pathways

Dysferlin operates within the calcium-dependent membrane repair pathway and the broader muscular dystrophy pathway. It interacts importantly with proteins such as annexins and actin that aid in membrane repair processes. Dysferlin's role in cellular repair connects it to pathways related to muscle membrane dynamics and stress response further highlighting its function in muscle health and recovery.

Mutations or deficiencies in dysferlin are linked to muscular dystrophies such as limb-girdle muscular dystrophy type 2B and Miyoshi myopathy. These conditions are characterized by progressive muscle weakness and wasting. The link between dysferlin's function and these disorders is also seen in its association with proteins like calpain 3 which also plays a role in muscle maintenance. Understanding the line of interaction between dysferlin and these proteins offers insights into potential therapeutic targets for these conditions.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (By similarity).
See full target information DYSF
style
left-column
style
left-column
style
right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com