Alexa Fluor® 488 Anti-Cytokeratin 8 抗体 [EP1628Y]

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (AB192467)

Flow cytometry analysis of HeLa cells stained with ab192467. The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab192467, 1/500 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor^® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• ICC

Lab

Immunocytochemistry - Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (AB192467)

Immunofluorescence staining of cytokeratin 8 in HeLa cells using ab192467. The cells were fixed with 4% formaldehyde (10 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab192647 at a working dilution of 1 in 100 (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1 μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with AlexaFluor^® 594 Goat anti-mouse IgG (H&L - preadsorbed) (ab150120) at 2 μg/ml (shown in pseudo-color red).

Nuclear DNA was labeled in blue with DAPI.

This product gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.

Image was taken with a Confocal microscope (Leica-microsystems, TCS SP8)

• ICC

PubMed

Immunocytochemistry - Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (AB192467)

Immunofluorescence staining in human mammary organoids of cytokeratin 8 using ab192467 (green), cytokeratin 14 using ab206100 (red), and actin (white). Cells were fixed with 4% paraformaldehyde for 20 minutes at room temperature and permeabilized with 0.5% Triton X-100 for 10 minutes at 4 °C. Primary antibodies incubated overnight at 4 °C. Nuclear DNA was labelled with DAPI (blue). Scale bar = 100 μm. Organoids were imaged by confocal microscopy.

Rosenbluth J et al., Nat Commun, 11(1), 1711. Fig 1c.; doi: 10.1038/s41467-020-15548-7. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

• ICC

PubMed

Immunocytochemistry - Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (AB192467)

Immunofluorescence analysis of mouse postate tumor samples labelling cytokeratin 8 (green) with ab192467 at 1/100 dilution. SYP was also stained using ab206870 (red). Cells were fixed with formalin and embedded in paraffin. Sections were blocked with PBS-Tween (0.1%) containing 5% of BSA. Primary conjugated antibodies were simultaneously incubated overnight at 4°C. Nuclear DNA was labelled with DAPI (blue). Scale bar = 100 μm.

Sulsenti R et al; Front Immunol. 2021 Mar 2;12:622001. doi: 10.3389/fimmu.2021.622001. Reproduced under the Creative Commons license: https://creativecommons.org/licenses/by/4.0/

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PubMed

Immunocytochemistry - Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (AB192467)

Immunofluorescence analysis of mouse myoepithelial cells labelling KRT8 (right) with ab192467 at 1/100 dilution. The tissue was fixed in 10% neutral buffered formalin overnight. Paraffin embedding and sectioning were performed by the Rodent Histopathology Core at Harvard Medical School. Scale bar, 10 μm.

Li CM et al; Cell Rep. 2020 Dec 29;33(13):108566. doi: 10.1016/j.celrep.2020.108566. Reproduced under the Creative Commons license: https://creativecommons.org/licenses/by/4.0/