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Alexa Fluor® 488 Anti-Cytochrome C 抗体 [37BA11] (ab154476)

Submit a review Submit a question References (3)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [37BA11] to Cytochrome C
  • Suitable for: Flow Cyt (Intra), ICC/IF
  • Reacts with: Mouse, Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 HRP Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11]
    Cytochrome C 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 488 Mouse monoclonal [37BA11] to Cytochrome C

  • 由来種

    Mouse

  • 標識

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IFmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Caenorhabditis elegans

  • 免疫原

    Full length native protein (purified) corresponding to Cow Cytochrome C.

  • 特記事項

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154476の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
Use a concentration of 0.2 µg/ml.

ab171464 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF
Use a concentration of 1 µg/ml.
特記事項
Flow Cyt (Intra)
Use a concentration of 0.2 µg/ml.

ab171464 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF
Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能

    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

  • 関連疾患

    Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.

  • 配列類似性

    Belongs to the cytochrome c family.

  • 翻訳後修飾

    Binds 1 heme group per subunit.

  • 細胞内局在

    Mitochondrion matrix.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CYC antibody
    • CYC_HUMAN antibody
    • CYCS antibody
    • Cytochrome c antibody
    • Cytochrome c somatic antibody
    • HCS antibody
    • THC4 antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
    Immunocytochemistry with HeLa cells (100x) were stained with Anti-Cytochrome C Alexa-488 antibody (1.0 µg/mL, ab154476) in green and DAPI in blue, as a nuclear stain.
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
    Immunocytochemistry with HDFn (100x) cells were stained with Anti-Cytochrome C Alexa-488 antibody (1.0 µg/mL, ab154476) in green, Anti-HSP60 (1/1000, ab46798) as red, and DAPI in blue, as a nuclear stain. Secondary antibody used was goat anti-rabbit dyelight-594 (1/1000, ab96897).
  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
    Flow Cytometry for Anti-Cytochrome C Alexa-488 antibody (ab154476) stained HeLa cells. Flow cytometry results of unstained cells (black) and 0.2 µg/mL Cytochrome C Alexa-488 (red) stained cells.

参考文献 (3)

ab154476 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab154476 は 3 報の論文で使用されています。

  • Zhou H  et al. The oncolytic compound LTX-401 targets the Golgi apparatus. Cell Death Differ 23:2031-2041 (2016). PubMed: 27588704
  • Tian XF  et al. PACE4 regulates apoptosis in human pancreatic cancer Panc-1 cells via the mitochondrial signaling pathway. Mol Med Rep 14:5205-5210 (2016). PubMed: 27779720
  • Zhou H  et al. The oncolytic peptide LTX-315 kills cancer cells through Bax/Bak-regulated mitochondrial membrane permeabilization. Oncotarget 6:26599-614 (2015). PubMed: 26378049

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