Alexa Fluor® 488 Anti-CD86 抗体 [EPR21962] (ab290990)
![Immunocytochemistry - Alexa Fluor® 488 Anti-CD86 antibody [EPR21962] (ab290990)](https://www.abcam.co.jp/ps/products/290/ab290990/Images/ab290990-484948-anti-CD86-antibody-EPR21962-ICC-human-raji.jpg "Immunocytochemistry - Alexa Fluor® 488 Anti-CD86 antibody [EPR21962] (ab290990)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR21962] to CD86
- Suitable for: ICC, Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 488 Anti-CD86 antibody [EPR21962]
CD86 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 488 Rabbit monoclonal [EPR21962] to CD86 -
由来種
Rabbit -
標識
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
アプリケーション
適用あり: ICC, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- ICC: Raji (human Burkitt's lymphoma B lymphocytes) Flow Cyt: Raji (Human Burkitt's lymphoma B lymphocytes)
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68.98% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR21962 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab290990の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
1/50.
|
|
| Flow Cyt |
1/500.
|
| 特記事項 |
|---|
|
ICC
1/50. |
|
Flow Cyt
1/500. |
ターゲット情報
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機能
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. -
組織特異性
Expressed by activated B-lymphocytes and monocytes. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 942 Human
- Omim: 601020 Human
- SwissProt: P42081 Human
- Unigene: 171182 Human
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別名
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
see all
画像
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Immunocytochemistry - Alexa Fluor® 488 Anti-CD86 antibody [EPR21962] (ab290990)
Immunofluorescent analysis of 4% Paraformaldehyde fixed Raji (human Burkitt's lymphoma B lymphocytes) permeabilized with 0.1% TritonX-100 labelling CD86 with ab290990 at 1/50 dilution, (Green). Confocal image showing membranous staining in Raji cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (shown in Red) was used to counterstain tubulin at 1/200 dilution. The nuclear counterstain was DAPI (Blue).
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![Flow Cytometry - Alexa Fluor® 488 Anti-CD86 antibody [EPR21962] (ab290990)](https://www.abcam.co.jp/ps/products/290/ab290990/Images/ab290990-484947-anti-CD86-antibody-EPR21962-flow-cytometry-human-raji.jpg)
Flow Cytometry - Alexa Fluor® 488 Anti-CD86 antibody [EPR21962] (ab290990)Flow cytometric overlay histogram showing Raji (Human Burkitt's lymphoma B lymphocytes) labelling CD86 with ab290990 at 1/500 dilution (Red). Isotype control: Rabbit monoclonal isotype control - Alexa Fluor® 488 (Black). Unlabelled control: Cells without incubation with primary antibody and secondary antibody (Blue).
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![Alexa Fluor® 488 Anti-CD86 antibody [EPR21962] (ab290990)](https://www.abcam.co.jp/ps/products/290/ab290990/Images/ab290990-484825-benefitsofrecombinantantibodies.jpg)
Alexa Fluor® 488 Anti-CD86 antibody [EPR21962] (ab290990)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab290990 は 2 報の論文で使用されています。
- Tan X et al. M2 macrophage-derived paracrine factor TNFSF13 affects the fibrogenic alterations in endothelial cells and cardiac fibroblasts by mediating the NF-κB and Akt pathway. J Biochem Mol Toxicol 38:e23707 (2024). PubMed: 38622979
- Tan X et al. A HER2-targeted antibody-novel DNA topoisomerase I inhibitor conjugate induces durable adaptive antitumor immunity by activating dendritic cells. MAbs 15:2220466 (2023). PubMed: 37314961