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AB302971

Alexa Fluor® 488 Anti-CD40 抗体 [EPR20735]

Alexa Fluor® 488 Anti-CD40 antibody [EPR20735]

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Rabbit Recombinant Monoclonal CD40 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt, IHC-P and reacts with Human samples.

別名を表示する

CD40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD40 antibody [EPR20735] (AB302971)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD40 antibody [EPR20735] (AB302971)

Immunocytochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) staining CD40 using ab302971 at a 1/50 dilution (10 μg/ml) (Green). Nuclear counterstain is DAPI (Blue). Counterstained with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (2.5 μg/ml) (Red). Confocal image showing membranous and weakly cytoplasmic staining in Raji cells. Negative control : Jurkat (PMID : 10498643). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry - Alexa Fluor® 488 Anti-CD40 antibody [EPR20735] (AB302971)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Anti-CD40 antibody [EPR20735] (AB302971)

Flow Cytometry analysis of Jurkat (human T cell leukemia T lymphocyte) (Left panel) and Raji (human Burkitt's lymphoma B lymphocyte) (Right panel) stained for CD40 using ab302971 at a 1/500 dilution (0.1 μg) (Red) compared to Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Gated on viable cells. Negative control : Jurkat (PMID : 10498643).

関連する標識済み抗体及び組成の異なる製品 (7)

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    Anti-CD40 antibody [EPR20735] - BSA and Azide free

  • Unconjugated

    Anti-CD40 antibody [EPR20735]

  • 660 APC

    APC Anti-CD40 antibody [EPR20735]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD40 antibody [EPR20735]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD40 antibody [EPR20735]

  • 519 FITC

    FITC Anti-CD40 antibody [EPR20735]

  • 578 PE

    PE Anti-CD40 antibody [EPR20735]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20735

アイソタイプ

IgG

標識

Alexa Fluor® 488

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

No

交差種

Human, Human

アプリケーション

ICC/IF, IHC-P, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

Receptor for TNFSF5/CD40LG (PubMed : 31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
See full target information CD40

Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com