Alexa Fluor® 488 Anti-CD30 抗体 [EPR24238-228] (ab300161)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR24238-228] to CD30
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 488 Anti-CD30 antibody [EPR24238-228]
CD30 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 488 Rabbit monoclonal [EPR24238-228] to CD30 -
由来種
Rabbit -
標識
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
アプリケーション
適用あり: ICC/IFmore details
適用なし: Flow Cyt -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- ICC/IF: KARPAS-299 (Human anaplastic large cell lymphoma cell line).
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR24238-228 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300161の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/50.
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特記事項 |
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ICC/IF
1/50. |
ターゲット情報
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機能
Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. -
配列類似性
Contains 6 TNFR-Cys repeats. -
翻訳後修飾
Phosphorylated on serine and tyrosine residues. -
細胞内局在
Cytoplasm and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 943 Human
- Omim: 153243 Human
- SwissProt: P28908 Human
- Unigene: 1314 Human
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別名
- CD 30 antibody
- CD30 antibody
- CD30 antigen antibody
see all
画像
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized KARPAS-299 (human anaplastic large cell lymphoma cell line) labelling CD30 with ab300161 at 1/50 dilution (Green). Confocal image showing membranous staining in KARPAS-299 cell line. The nuclear counterstain is DAPI (Blue).
Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (Red) at 1/200 dilution.
Negative control: HeLa (PMID: 11004694).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab300161 は論文での使用が確認できていません。