Alexa Fluor® 488 Anti-CD24 抗体 [EPR19925] (ab300545)
![Flow Cytometry - Alexa Fluor® 488 Anti-CD24 antibody [EPR19925] (AB300545)](https://www.abcam.co.jp/ps/products/300/ab300545/Images/AB300545-1-anti-cd24-antibody-epr19925-flow-cytometry-mdamb231.jpg "Flow Cytometry - Alexa Fluor® 488 Anti-CD24 antibody [EPR19925] (AB300545)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR19925] to CD24
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 488 Anti-CD24 antibody [EPR19925]
CD24 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 488 Rabbit monoclonal [EPR19925] to CD24 -
由来種
Rabbit -
標識
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
特異性
Not suitable for Immunocytochemistry (ICC)
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アプリケーション
適用あり: Flow Cytmore details
適用なし: ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Flow cytometry: MCF7 (human breast adenocarcinoma epithelial cells).
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特記事項
Note:
Not suitable for Immunocytochemistry (ICC)
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR19925 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300545の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
1/50.
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| 特記事項 |
|---|
|
Flow Cyt
1/50. |
ターゲット情報
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機能
Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. -
組織特異性
B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells. -
関連疾患
Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface. -
配列類似性
Belongs to the CD24 family. -
翻訳後修飾
Extensively O-glycosylated. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 100133941 Human
- Omim: 600074 Human
- SwissProt: P25063 Human
- Unigene: 644105 Human
- Unigene: 721443 Human
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別名
- CD 24 antibody
- CD24 antibody
- CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
see all
画像
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Flow Cytometry - Alexa Fluor® 488 Anti-CD24 antibody [EPR19925] (AB300545)
Flow cytometric analysis of MDA-MB-231 (human breast adenocarcinoma epithelial cell, (Left))(Negative control) / MCF7 (human breast adenocarcinoma epithelial cell, (Right)) cells labeling CD24 with ab300545 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal isotype control - Alexa Fluor® 488 (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Gated on viable cells. Negative control: MDA-MB-231 (PMID: 18433506 and 22934288).
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![Alexa Fluor® 488 Anti-CD24 antibody [EPR19925] (AB300545)](https://www.abcam.co.jp/ps/products/300/ab300545/Images/AB300545-2-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 488 Anti-CD24 antibody [EPR19925] (AB300545)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab300545 は論文での使用が確認できていません。