AB187575

Alexa Fluor® 488 Anti-CD105 抗体 [MEM226], prediluted

Name: Alexa Fluor® 488 Anti-CD105 抗体 [MEM226], prediluted (ab187575) | Abcam
Brand: Abcam Limited
SKU: AB187575
Price: 24000 JPY
Availability: InStock

Alexa Fluor® 488 Anti-CD105 antibody [MEM226]

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(2 Publications)

Anti-CD105 antibody [MEM226] conjugated to Alexa Fluor® 488 - suitable for flow cytometry, and reacts with human samples. Clone [MEM226] is a mouse monoclonal antibody.

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別名を表示する

CD105, END, ENG, Endoglin

1 Images

Flow Cytometry - Alexa Fluor® 488 Anti-CD105 antibody [MEM226] (AB187575)

Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 488 Anti-CD105 antibody [MEM226] (AB187575)

Surface staining of HUVEC (human umbilical vein endothelial cells) with ab187575 at 1/25 dilution

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

MEM226

アイソタイプ

IgG2a

標識

Alexa Fluor® 488

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

Recombinant Full Length Protein corresponding to Human ENG.

P17813

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Use 4 μl for 100 μl of Human whole blood cells in suspension.</p><p>ab171464 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Size-exclusion chromatography

精製に関する特記事項

ab187575 is conjugated with Alexa Fluor® 488 under optimum conditions and the conjugate purified by size-exclusion chromatography and adjusted for direct use.

バッファー組成

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.

Biological function summary

Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.

Pathways

CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.

CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed : 21737454, PubMed : 23300529). Required for normal structure and integrity of adult vasculature (PubMed : 7894484). Regulates the migration of vascular endothelial cells (PubMed : 17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed : 1692830). Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529, PubMed : 8370410). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529).

See full target information ENG

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39519007

2024

Development and Characterization of a Human Mammary Epithelial Cell Culture Model for the Blood-Milk Barrier-A Contribution from the ConcePTION Project.

Applications

Unspecified application

Species

Unspecified reactive species

Debora La Mantia,Nina Nauwelaerts,Chiara Bernardini,Augusta Zannoni,Roberta Salaroli,Qi Lin,Isabelle Huys,Pieter Annaert,Monica Forni

NPJ Regenerative medicine 9:4 PubMed38242900

2024

hUC-MSCs-derived MFGE8 ameliorates locomotor dysfunction via inhibition of ITGB3/ NF-κB signaling in an NMO mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Huiming Xu,Wei Jiang,Xuejia Li,Jiaohua Jiang,Shabbir Khan Afridi,Longhui Deng,Rui Li,Ermei Luo,Zhaoqing Zhang,Yu-Wen Alvin Huang,Yaxiong Cui,Kwok-Fai So,Haijia Chen,Wei Qiu,Changyong Tang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-CD105 抗体 [MEM226], prediluted

Alexa Fluor® 488 Anti-CD105 antibody [MEM226]

Flow Cytometry - Alexa Fluor® 488 Anti-CD105 antibody [MEM226] (AB187575)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

Abcam product promise