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リコンビナントRabMAb

Alexa Fluor® 488 Anti-Caveolin-1 抗体 [E249] - Caveolae Marker (ab185043)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab185043)
  • Alexa Fluor® 488 Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab185043)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [E249] to Caveolin-1 - Caveolae Marker
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 750 Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 488 Anti-Caveolin-1 antibody [E249] - Caveolae Marker
    Caveolin-1 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 488 Rabbit monoclonal [E249] to Caveolin-1 - Caveolae Marker

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • アプリケーション

    適用あり: ICC/IFmore details

  • 種交差性

    交差種: Human
    非交差種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: HeLa cells.

  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185043の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/50.
特記事項
ICC/IF
1/50.

ターゲット情報

  • 機能

    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.

  • 組織特異性

    Expressed in muscle and lung, less so in liver, brain and kidney.

  • 関連疾患

    Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.

  • 配列類似性

    Belongs to the caveolin family.

  • 翻訳後修飾

    The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.

  • 細胞内局在

    Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Information by UniProt

  • 参照データベース

    • 別名

      • BSCL3 antibody
      • CAV antibody
      • CAV1 antibody
      • CAV1_HUMAN antibody
      • caveolae protein, 22 kD antibody
      • caveolin 1 alpha isoform antibody
      • caveolin 1 beta isoform antibody
      • Caveolin 1 caveolae protein 22kDa antibody
      • Caveolin-1 antibody
      • Caveolin1 antibody
      • cell growth-inhibiting protein 32 antibody
      • CGL3 antibody
      • LCCNS antibody
      • MSTP085 antibody
      • OTTHUMP00000025031 antibody
      • PPH3 antibody
      • VIP 21 antibody
      • VIP21 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab185043)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab185043)

      ab185043 staining Caveolin-1 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab185043 at a working dilution of 1 in 50 (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor® 594 Goat anti-Mouse secondary (ab150120) at 2μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 488 Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab185043)
      Alexa Fluor® 488 Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab185043)

    参考文献 (2)

    ab185043 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab185043 は 2 報の論文で使用されています。

    • Khalin I  et al. Size-Selective Transfer of Lipid Nanoparticle-Based Drug Carriers Across the Blood Brain Barrier Via Vascular Occlusions Following Traumatic Brain Injury. Small 18:e2200302 (2022). PubMed: 35384294
    • Xin X  et al. Drug-delivering-drug platform-mediated potent protein therapeutics via a non-endo-lysosomal route. Theranostics 8:3474-3489 (2018). PubMed: 30026860

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