Alexa Fluor® 488 Anti-c-Myc (phospho T58) 抗体 [EPR17923] (ab300546)
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-c-Myc (phospho T58) antibody [EPR17923] (AB300546)](https://www.abcam.co.jp/ps/products/300/ab300546/Images/AB300546-1-anti-cmyc-phospho-t58-antibody-epr17923-flow-cytometry-hela1.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-c-Myc (phospho T58) antibody [EPR17923] (AB300546)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR17923] to c-Myc (phospho T58)
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 488 Anti-c-Myc (phospho T58) antibody [EPR17923]
c-Myc 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 488 Rabbit monoclonal [EPR17923] to c-Myc (phospho T58) -
由来種
Rabbit -
標識
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
アプリケーション
適用あり: Flow Cyt (Intra)more details
適用なし: ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Flow Cyt (Intra): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 200nM Calyculin A and 1uM Okadaic Acid.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR17923 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free (ab236021)
- PE Anti-c-Myc (phospho T58) antibody [EPR17923] (ab237249)
- APC Anti-c-Myc (phospho T58) antibody [EPR17923] (ab310838)
- Alexa Fluor® 594 Anti-c-Myc (phospho T58) antibody [EPR17923] (ab311655)
- Alexa Fluor® 568 Anti-c-Myc (phospho T58) antibody [EPR17923] (ab312928)
- Alexa Fluor® 555 Anti-c-Myc (phospho T58) antibody [EPR17923] (ab313141)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300546の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/500. |
ターゲット情報
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機能
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. -
関連疾患
Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻訳後修飾
Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. -
細胞内局在
Nucleus > nucleoplasm. Nucleus > nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 4609 Human
- Omim: 190080 Human
- SwissProt: P01106 Human
- Unigene: 202453 Human
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製品の状態
c-Myc is also expressed in the cytoplasm. -
別名
- AU016757 antibody
- Avian myelocytomatosis viral oncogene homolog antibody
- bHLHe39 antibody
see all
画像
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Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-c-Myc (phospho T58) antibody [EPR17923] (AB300546)
Flow cytometric (Intracellular) analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells treated with 200nM Calyculin A and 1µM Okadaic Acid for 60 min (Red) / Untreated control (Green) cells labeling c-Myc (phospho T58) with ab300546 at 1/500 dilution (0.1µg) (Red) and Green (Red) compared with a Rabbit monoclonal isotype control - Alexa Fluor® 488 (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).
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![Alexa Fluor® 488 Anti-c-Myc (phospho T58) antibody [EPR17923] (AB300546)](https://www.abcam.co.jp/ps/products/300/ab300546/Images/AB300546-2-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 488 Anti-c-Myc (phospho T58) antibody [EPR17923] (AB300546)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab300546 は論文での使用が確認できていません。