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リコンビナントRabMAb

Alexa Fluor® 488 Anti-c-Kit 抗体 [EPR24708-25] (ab283322)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-c-Kit antibody [EPR24708-25] (ab283322)
  • Flow Cytometry - Alexa Fluor® 488 Anti-c-Kit antibody [EPR24708-25] (ab283322)
  • Flow Cytometry - Alexa Fluor® 488 Anti-c-Kit antibody [EPR24708-25] (ab283322)
  • Alexa Fluor® 488 Anti-c-Kit antibody [EPR24708-25] (ab283322)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR24708-25] to c-Kit
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Mouse
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free Carrier Free Unconjugated Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 488 Anti-c-Kit antibody [EPR24708-25]
    c-Kit 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 488 Rabbit monoclonal [EPR24708-25] to c-Kit

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • アプリケーション

    適用あり: ICC/IF, Flow Cytmore details

  • 種交差性

    交差種: Mouse

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: P815 (mouse mastocytoma mast cells) Flow Cyt: Mouse bone marrow cells.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab283322の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/50.
Flow Cyt
1/500.
特記事項
ICC/IF
1/50.
Flow Cyt
1/500.

ターゲット情報

  • 機能

    Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

  • 組織特異性

    Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

  • 関連疾患

    Piebald trait
    Gastrointestinal stromal tumor
    Testicular germ cell tumor
    Leukemia, acute myelogenous

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.
    Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.

  • 細胞内局在

    Cell membrane and Cytoplasm. Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C Kit antibody
      • c-Kit antibody
      • c-Kit Ligand antibody
      • CD117 antibody
      • Kit antibody
      • Kit Ligand antibody
      • KIT oncogene antibody
      • KIT proto oncogene receptor tyrosine kinase antibody
      • KIT_HUMAN antibody
      • Mast cell growth factor receptor antibody
      • Mast/stem cell growth factor receptor Kit antibody
      • MGF antibody
      • p145 c-kit antibody
      • PBT antibody
      • Piebald trait protein antibody
      • Proto oncogene c Kit antibody
      • Proto oncogene tyrosine protein kinase Kit antibody
      • Proto-oncogene c-Kit antibody
      • SCF Receptor antibody
      • SCFR antibody
      • soluble KIT variant 1 antibody
      • Steel Factor Receptor antibody
      • Stem cell factor receptor antibody
      • tyrosine protein kinase Kit antibody
      • Tyrosine-protein kinase Kit antibody
      • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog antibody
      • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody
      • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-c-Kit antibody [EPR24708-25] (ab283322)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-c-Kit antibody [EPR24708-25] (ab283322)

      Immunofluorescent analysis of 4% Paraformaldehyde P815 (mouse mastocytoma mast cells labelling c-Kit with ab283322 at 1/50 dilution, (green). Confocal image showing membranous staining on P815 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      The negative control is as follows:
      -ve control: ab283322 at 1/50 dilution in NIH/3T3 cells (mouse embryonic fibroblasts) showing negative staining.

    • Flow Cytometry - Alexa Fluor® 488 Anti-c-Kit antibody [EPR24708-25] (ab283322)
      Flow Cytometry - Alexa Fluor® 488 Anti-c-Kit antibody [EPR24708-25] (ab283322)

      Flow cytometric scatterplot showing Mouse bone marrow cell line (Right) labelling c-Kit with ab283322 at 1/500 dilution. Isotype control antibody (Left) was Rabbit monoclonal isotype control - Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody.

      Cells were stained with anti-CD45R/B220 conjugated to Alexa Fluor®647.

      Gated on viable cells.

    • Flow Cytometry - Alexa Fluor® 488 Anti-c-Kit antibody [EPR24708-25] (ab283322)
      Flow Cytometry - Alexa Fluor® 488 Anti-c-Kit antibody [EPR24708-25] (ab283322)

      Flow cytometric scatterplot showing Mouse bone marrow cell line (Right) labelling c-Kit with ab283322 at 1/500 dilution. Isotype control antibody (Left) was Rabbit monoclonal isotype control - Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody.

      Cells were stained with anti-CD34 conjugated to BV421.

      Gated on viable cells.

    • Alexa Fluor® 488 Anti-c-Kit antibody [EPR24708-25] (ab283322)
      Alexa Fluor® 488 Anti-c-Kit antibody [EPR24708-25] (ab283322)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab283322 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab283322 は論文での使用が確認できていません。

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