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AB284135

Alexa Fluor® 488 Anti-Aquaporin 4 抗体 [EPR24281-65]

Alexa Fluor® 488 Anti-Aquaporin 4 antibody [EPR24281-65]

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(3 Publications)

Rabbit Recombinant Monoclonal Aquaporin 4 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P, IHC-Fr, Flow Cyt (Intra), ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

別名を表示する

Aquaporin-4, AQP-4, Mercurial-insensitive water channel, WCH4, MIWC, AQP4

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Aquaporin 4 antibody [EPR24281-65] (AB284135)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Aquaporin 4 antibody [EPR24281-65] (AB284135)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Aquaporin 4 with ab284135 at 1/100 (5.0 ug/ml) dilution. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab284135 at 1/100 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Aquaporin 4 antibody [EPR24281-65] (AB284135)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Aquaporin 4 antibody [EPR24281-65] (AB284135)

Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labeling Aquaporin 4 with ab284135 at 1/100 (5.0 ug/ml) dilution. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab284135 at 1/100 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Aquaporin 4 antibody [EPR24281-65] (AB284135)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Aquaporin 4 antibody [EPR24281-65] (AB284135)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling Aquaporin 4 with ab284135 at /100 (5.0 ug/ml) dilution followed by at dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Aquaporin 4 antibody [EPR24281-65] (AB284135)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Aquaporin 4 antibody [EPR24281-65] (AB284135)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Aquaporin 4 with ab284135 at 1/100 (5.0 ug/ml) dilution. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab284135 at 1/100 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Aquaporin 4 antibody [EPR24281-65] (AB284135)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Aquaporin 4 antibody [EPR24281-65] (AB284135)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Aquaporin 4 with ab284135 at 1/100 (5.0 ug/ml) dilution. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab284135 at 1/100 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Aquaporin 4 antibody [EPR24281-65] (AB284135)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Aquaporin 4 antibody [EPR24281-65] (AB284135)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling Aquaporin 4 with ab284135 at /100 (5.0 ug/ml) dilution followed by at dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Aquaporin 4 antibody [EPR24281-65] (AB284135)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Aquaporin 4 antibody [EPR24281-65] (AB284135)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Aquaporin 4 with ab284135 at 1/100 (5.0 ug/ml) dilution followed by a ready to use negative control. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab284135 at 1/100 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

関連する標識済み抗体及び組成の異なる製品 (4)

  • Unconjugated

    Anti-Aquaporin 4 antibody [EPR24281-65]

  • Carrier free

    Anti-Aquaporin 4 antibody [EPR24281-65] - BSA and Azide free

  • Carrier free

    Anti-Aquaporin 4 antibody [EPR24281-85] - BSA and Azide free (Detector)

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Aquaporin 4 antibody [EPR24281-65]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR24281-65

アイソタイプ

IgG

標識

Alexa Fluor® 488

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

No

交差種

Rat, Human, Mouse, Human, Mouse, Rat

アプリケーション

IHC-Fr, IHC-P, ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Assay Kits

AB290982

Human Aquaporin 4 ELISA Kit - Extracellular

Recombinant|SimpleStep

Sandwich ELISA - Human Aquaporin 4 ELISA Kit - Extracellular (AB290982)

  • 0
  • 0
Primary Antibodies

AB302977

Alexa Fluor® 488 Anti-GFAP antibody [EP672Y] - Astrocyte marker

BOND RX™ Validated|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-GFAP antibody [EP672Y] - Astrocyte marker (AB302977)

  • 3
  • 2
Primary Antibodies

AB225261

Alexa Fluor® 647 Anti-Iba1 antibody [EPR16588] - Microglia marker

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Iba1 antibody [EPR16588] - Microglia marker (AB225261)

  • 0
  • 0
Primary Antibodies

AB275738

Alexa Fluor® 488 Anti-CD11c antibody [EP1347Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD11c antibody [EP1347Y] (AB275738)

  • 5
  • 2
Primary Antibodies

AB195020

Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915]

RabMAb|Recombinant|KO Validated|Trial Size

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB195020)

  • 4
  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Aquaporin 4 also known as AQP4 functions as a water channel protein that facilitates the transport of water across cell membranes. It is composed of approximately 323 amino acids resulting in a molecular mass of around 34-38 kDa. This target expresses most in the central nervous system especially in the astrocytes of the brain and spinal cord. Other expression sites include skeletal muscle kidneys and lungs. AQP4 is often studied using various tools including anti-aquaporin 4 antibodies and specific reagents like AQP4 Ab and anti-AQP4.
Biological function summary

Water balance and neural activity regulation are significantly influenced by aquaporin 4. This protein forms a part of the orthogonal array of particles (OAPs) in the plasma membrane which enhances its water permeability function. Through its concentration at the blood-brain barrier AQP4 contributes to maintaining brain homeostasis by regulating osmotic gradients and extracellular space ion concentrations. Its expression and activity may affect critical processes like neuroexcitation and neuroprotection.

Pathways

Aquaporin 4 actively engages in critical water transport and blood-brain barrier integrity pathways. The passive yet selective water transport facilitated by AQP4 impacts pathways including the transcellular pathway that helps in maintaining the homeostatic and osmotic balance important for brain function. Furthermore AQP4 acts in relation to other proteins such as aquaporin 1 and connexin 43 indicating its role in gap junction communication within the brain.

Aquaporin 4 is intricately linked to conditions like neuromyelitis optica (NMO) and cerebral edema. In NMO autoantibodies such as anti-AQP4 target the protein causing demyelination and inflammation of the optic nerve and spinal cord. In cases of cerebral edema an imbalance in AQP4 expression can disrupt water homeostasis leading to exacerbated swelling. AQP4's interaction with proteins like myelin basic protein in these diseases underlines its significance in neurodegeneration and inflammatory processes.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Forms a water-specific channel (PubMed : 19383790, PubMed : 7559426, PubMed : 8601457). Plays an important role in brain water homeostasis (PubMed : 37143309). It is involved in glymphatic solute transport and is required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability (By similarity).
See full target information AQP4
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40598893

2025

Elucidating brain transport pathways and cell type-dependent gene silencing of a durable lipid-siRNA conjugate administered into cerebrospinal fluid.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander G Sorets,Katrina R Schwensen,Nora Francini,Andrew Kjar,Adam M Abdulrahman,Alena Shostak,Ketaki A Katdare,Kathleen M Schoch,Rebecca P Cowell,Joshua C Park,Alexander P Ligocki,William T Ford,Lissa Ventura-Antunes,Ella N Hoogenboezem,Alex Prusky,Mark Castleberry,Danielle L Michell,Emma Fritsch,Sarah M Lyons,Timothy M Miller,Kasey C Vickers,Matthew S Schrag,Craig L Duvall,Ethan S Lippmann

Frontiers in pharmacology 15:1421635 PubMed39148543

2024

Angong Niuhuang Wan ameliorates LPS-induced cerebrovascular edema by inhibiting blood‒brain barrier leakage and promoting the membrane expression of AQP4.

Applications

Unspecified application

Species

Unspecified reactive species

Bo-Tong Liu,Quan Li,Kai Sun,Chun-Shui Pan,Xin-Mei Huo,Ping Huang,Li Yan,Qi-Hua He,Li-Jun Zhong,Yuan Wang,Meng-Lei Hu,An-Qing Li,Ying-Qian Jiao,Shuang Zhang,Xiao-Yi Wang,Jian Liu,Jing-Yan Han

Frontiers in cellular neuroscience 16:858347 PubMed35573835

2022

Centrin 2: A Novel Marker of Mature and Neoplastic Human Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Degl'Innocenti,Tino Emanuele Poloni,Valentina Medici,Luca Recupero,Claudia Dell'Amico,Eleonora Vannini,Ugo Borello,Chiara Maria Mazzanti,Marco Onorati,Maria Teresa Dell'Anno
View all publications
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Abcam product promise

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