Anti-Aldose reductase 抗体 (ab153897)
Key features and details
- Rabbit polyclonal to Aldose reductase
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Aldose reductase antibody
Aldose reductase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Aldose reductase -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Rabbit, Cow
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免疫原
Recombinant fragment corresponding to Human Aldose reductase aa 1-316.
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ポジティブ･コントロール
- 293T, A431, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells; Human colon carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153897の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 36 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 36 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. -
組織特異性
Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress. -
配列類似性
Belongs to the aldo/keto reductase family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 317748 Cow
- Entrez Gene: 231 Human
- Entrez Gene: 100009122 Rabbit
- Entrez Gene: 24192 Rat
- Omim: 103880 Human
- SwissProt: P16116 Cow
- SwissProt: P15121 Human
- SwissProt: P15122 Rabbit
see all -
別名
- ADR antibody
- AKR1B 1 antibody
- Akr1b1 antibody
see all
画像
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Western blot - Anti-Aldose reductase antibody (ab153897)All lanes : Anti-Aldose reductase antibody (ab153897) at 1/2500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Aldose reductase Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa -
Western blot - Anti-Aldose reductase antibody (ab153897)All lanes : Anti-Aldose reductase antibody (ab153897) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody (ab153897)Immunofluorescent analysis of methanol-fixed HeLa cells labeling Aldose reductase with ab153897 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody (ab153897)Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Aldose reductase with ab153897 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab153897 は 2 報の論文で使用されています。
- Zhang Z et al. Effect of AR gene-specific knockout on the process of radiation-induced pulmonary fibrosis and its mechanism. Cell Mol Biol (Noisy-le-grand) 69:156-162 (2023). PubMed: 37571887
- Chen X et al. AKR1B1 Upregulation Contributes to Neuroinflammation and Astrocytes Proliferation by Regulating the Energy Metabolism in Rat Spinal Cord Injury. Neurochem Res 43:1491-1499 (2018). PubMed: 29948725