Anti-Aldolase C 抗体 - Astrocyte Marker (ab87122)
Key features and details
- Rabbit polyclonal to Aldolase C - Astrocyte Marker
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Aldolase C antibody - Astrocyte Marker
Aldolase C 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Aldolase C - Astrocyte Marker -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow, Chimpanzee, Monkey
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab87122の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 39 kDa).
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 39 kDa). |
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ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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パスウェイ
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. -
配列類似性
Belongs to the class I fructose-bisphosphate aldolase family. - Information by UniProt
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参照データベース
- Entrez Gene: 493951 Chimpanzee
- Entrez Gene: 504584 Cow
- Entrez Gene: 230 Human
- Entrez Gene: 11676 Mouse
- Entrez Gene: 24191 Rat
- Omim: 103870 Human
- SwissProt: Q5R1X4 Chimpanzee
- SwissProt: P09972 Human
see all -
別名
- ALDC antibody
- ALDO C antibody
- aldoc antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Aldolase C antibody - Astrocyte Marker (ab87122)
ab87122 staining Fructose-bisphosphate aldolase C in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab87122 at 5µg/ml and ab4674, Chicken polyclonal to GFAP. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150176, Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-Aldolase C antibody - Astrocyte Marker (ab87122)All lanes : Anti-Aldolase C antibody - Astrocyte Marker (ab87122) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
The 44 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Mouse Fructose-bisphosphate aldolase C.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab87122 は 8 報の論文で使用されています。
- Wei J et al. Osteoblasts induce glucose-derived ATP perturbations in chondrocytes through noncontact communication. Acta Biochim Biophys Sin (Shanghai) 54:625-636 (2022). PubMed: 35593470
- Luo J et al. lncRNA GAS6-AS1 inhibits progression and glucose metabolism reprogramming in LUAD via repressing E2F1-mediated transcription of GLUT1. Mol Ther Nucleic Acids 25:11-24 (2021). PubMed: 34141461
- Ai LQ et al. Retinal blood vessel-origin yes-associated protein (YAP) governs astrocytic maturation via leukaemia inhibitory factor (LIF). Cell Prolif 53:e12757 (2020). PubMed: 31916327
- Zhang H et al. A role of low-density lipoprotein receptor-related protein 4 (LRP4) in astrocytic Aß clearance. J Neurosci N/A:N/A (2020). PubMed: 32457076
- Cardona C et al. Identification of the iduronate-2-sulfatase proteome in wild-type mouse brain. Heliyon 5:e01667 (2019). PubMed: 31193135
- Xiao ZD et al. Energy stress-induced lncRNA FILNC1 represses c-Myc-mediated energy metabolism and inhibits renal tumor development. Nat Commun 8:783 (2017). PubMed: 28978906
- Falcone C et al. Emx2 expression levels in NSCs modulate astrogenesis rates by regulating EgfR and Fgf9. Glia 63:412-22 (2015). PubMed: 25327963
- Li J et al. EZH2-mediated H3K27 trimethylation mediates neurodegeneration in ataxia-telangiectasia. Nat Neurosci 16:1745-53 (2013). PubMed: 24162653