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リコンビナントRabMAb

Anti-Aldolase B + Aldolase C 抗体 [EPR3138Y] (ab75751)

Reviews (2) Submit a question References (7)
Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
  • Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
  • Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3138Y] to Aldolase B + Aldolase C
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

APC Carrier Free HRP PE

製品の概要

  • 製品名

    Anti-Aldolase B + Aldolase C antibody [EPR3138Y]
    Aldolase B+Aldolase C 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR3138Y] to Aldolase B + Aldolase C

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt, WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human Aldolase B aa 250-350. The exact sequence is proprietary.
    Database link: P05062

  • ポジティブ･コントロール

    • IHC: Human, mouse and rat kidney tissues. WB: Human, mouse and rat kidney lysate. His-tagged human Aldolase B and C full-length recombinant protein.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab75751の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000. Predicted molecular weight: 39 kDa.

For unpurified use at 1/500 - 1/1000.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
特記事項
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000. Predicted molecular weight: 39 kDa.

For unpurified use at 1/500 - 1/1000.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

画像

  • Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
    Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
    All lanes : Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751) at 1/200 dilution

    Lane 1 : His-tagged human Aldolase A full-length recombinant protein
    Lane 2 : His-tagged human Aldolase B full-length recombinant protein
    Lane 3 : His-tagged human Aldolase C full-length recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?


    Exposure time: 40 seconds


    Blocking and diluting buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
    Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
    All lanes : Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751) at 1/1000 dilution (Purified)

    Lane 1 : Human kidney lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Rat kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    Blocking Buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Aldolase B with Purified ab75751 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Aldolase B with Purified ab75751 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Aldolase B with Purified ab75751 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
    Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)

参考文献 (7)

ab75751 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab75751 は 7 報の論文で使用されています。

  • Liu Y  et al. Intracellular pH dynamics regulates intestinal stem cell lineage specification. Nat Commun 14:3745 (2023). PubMed: 37353491
  • Niec RE  et al. Lymphatics act as a signaling hub to regulate intestinal stem cell activity. Cell Stem Cell 29:1067-1082.e18 (2022). PubMed: 35728595
  • Ramadan R  et al. The extracellular matrix controls stem cell specification and crypt morphology in the developing and adult mouse gut. Biol Open 11:N/A (2022). PubMed: 36350252
  • Fahrner M  et al. Proteome biology of primary colorectal carcinoma and corresponding liver metastases. Neoplasia 23:1240-1251 (2021). PubMed: 34768110
  • Krotenberg Garcia A  et al. Generation of mixed murine organoids to model cellular interactions. STAR Protoc 2:100997 (2021). PubMed: 34917977
  • Wang C  et al. Long-term supranutritional supplementation with selenate decreases hyperglycemia and promotes fatty liver degeneration by inducing hyperinsulinemia in diabetic db/db mice. PLoS One 9:e101315 (2014). WB . PubMed: 24983750
  • Tun HW  et al. Pathway signature and cellular differentiation in clear cell renal cell carcinoma. PLoS One 5:e10696 (2010). IHC-P ; Human . PubMed: 20502531

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Aldolase B + Aldolase C antibody [EPR3138Y]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse small intestine, 30um)
Permeabilization
Yes - 1% triton, 1hr
Specification
mouse small intestine, 30um
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Neta Felsenthal

Verified customer

投稿 Aug 02 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-Aldolase B + Aldolase C antibody [EPR3138Y]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Intestinal organoids)
Permeabilization
Yes - Triton X-100 0.5% in 1X PBS pH 7.4
Specification
Intestinal organoids
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Mahi Rahman

Verified customer

投稿 Apr 22 2021

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