Anti-ALDH7A1 抗体 (ab154264)
Key features and details
- Rabbit polyclonal to ALDH7A1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ALDH7A1 antibody
ALDH7A1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ALDH7A1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow
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免疫原
Recombinant fragment, corresponding to a region within amino acids 261-479 of Human ALDH7A1.
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ポジティブ･コントロール
- A431, H1299, HeLa, HepG2 and mouse liver lysates; HeLa cells; MCF7 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154264の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 58 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 507477 Cow
- Entrez Gene: 501 Human
- Entrez Gene: 110695 Mouse
- Omim: 107323 Human
- SwissProt: Q2KJC9 Cow
- SwissProt: P49419 Human
- SwissProt: Q9DBF1 Mouse
- Unigene: 483239 Human
see all -
別名
- 26g turgor protein homolog antibody
- AL7A1_HUMAN antibody
- Aldehyde dehydrogenase 7 A1 antibody
see all
画像
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Western blot - Anti-ALDH7A1 antibody (ab154264)Anti-ALDH7A1 antibody (ab154264) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 58 kDa
7.5% SDS PAGE -
Western blot - Anti-ALDH7A1 antibody (ab154264)Anti-ALDH7A1 antibody (ab154264) at 1/10000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 58 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-ALDH7A1 antibody (ab154264)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling ALDH7A1 with ab154264 at 1/200 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (ab154264)Immunohistochemical analysis of paraffin-embedded MCF7 xenograft labeling ALDH7A1 with ab154264 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154264 は 1 報の論文で使用されています。
- Struebing FL et al. Genomic loci modulating retinal ganglion cell death following elevated IOP in the mouse. Exp Eye Res 169:61-67 (2018). IHC-Fr ; Mouse . PubMed: 29421330