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リコンビナントRabMAb

Anti-ALDH4A1/P5CDH 抗体 [EPR14288(B)] (ab181256)

Submit a review Submit a question References (1)
Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)
  • Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)
  • Immunocytochemistry/ Immunofluorescence - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)
  • Immunoprecipitation - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)
  • Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14288(B)] to ALDH4A1/P5CDH
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ALDH4A1/P5CDH antibody [EPR14288(B)]
    ALDH4A1/P5CDH 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14288(B)] to ALDH4A1/P5CDH

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IF, IPmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HepG2, HeLa and K562 cell lysates; Human fetal heart and liver lysates; Human liver and kidney tissues; HepG2 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181256の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/1000.
IP
1/40 - 1/60.
特記事項
WB
1/10000 - 1/50000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/1000.
IP
1/40 - 1/60.

ターゲット情報

  • 機能

    Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.

  • 組織特異性

    Highest expression is found in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas.

  • パスウェイ

    Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.

  • 関連疾患

    Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2) [MIM:239510]. HP-2 is characterized by the accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. The disorder may be causally related to neurologic manifestations, including seizures and mental retardation.

  • 配列類似性

    Belongs to the aldehyde dehydrogenase family.

  • 細胞内局在

    Mitochondrion matrix.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AL4A1_HUMAN antibody
    • aldehyde dehydrogenase 4 antibody
    • aldehyde dehydrogenase 4 family, member A1 antibody
    • Aldehyde dehydrogenase family 4 member A1 antibody
    • Aldehyde dehydrogenase, family 4, subfamily A, member 1 antibody
    • ALDH4 antibody
    • aldh4a1 antibody
    • Delta 1 pyrroline 5 carboxylate dehydrogenase, mitochondrial antibody
    • Delta-1-pyrroline-5-carboxylate dehydrogenase antibody
    • L-glutamate gamma-semialdehyde dehydrogenase antibody
    • mitochondrial antibody
    • mitochondrial delta-1-pyrroline 5-carboxylate dehydrogenase antibody
    • P5C dehydrogenase antibody
    • P5CD antibody
    • P5CDh antibody
    • P5CDhL antibody
    • P5CDhS antibody
    • Pyrroline-5-carboxylate dehydrogenase antibody
    • RP11 128M10.1 antibody
    see all

画像

  • Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)
    Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)
    All lanes : Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256) at 1/20000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

  • Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)
    Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)
    Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256) at 1/20000 dilution + Human fetal liver tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Developed using the ECL technique.

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)

    Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling ALDH4A1/P5CDH with ab181256 at a 1/250 dilution. Counter stained hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)

    Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling ALDH4A1/P5CDH with ab181256 at a 1/250 dilution. Counter stained hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)
    Immunocytochemistry/ Immunofluorescence - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)

    Immunofluorescence analysis of, paraformaldehyde-fixed, HepG2 cells labeling ALDH4A1/P5CDH with ab181256 at a 1/1000 dilution. As secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) was used at a 1/200 dilution. In blue DAPI staining.

  • Immunoprecipitation - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)
    Immunoprecipitation - Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)

    Western blot analysis on Immunoprecipitation from HepG2 cell lysate; showing ALDH4A1/P5CDH, using ab181256 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

  • Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)
    Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256)

参考文献 (1)

ab181256 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab181256 は 1 報の論文で使用されています。

  • Yen CA & Curran SP Incomplete proline catabolism drives premature sperm aging. Aging Cell 20:e13308 (2021). PubMed: 33480139

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