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リコンビナントRabMAb

Anti-ALDH4A1/P5CDH 抗体 [EPR14287] (ab185208)

Submit a review Submit a question References (5)
Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
  • Immunocytochemistry/ Immunofluorescence - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
  • Flow Cytometry (Intracellular) - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
  • Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
  • Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14287] to ALDH4A1/P5CDH
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ALDH4A1/P5CDH antibody [EPR14287]
    ALDH4A1/P5CDH 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14287] to ALDH4A1/P5CDH

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human fetal hear and HepG2 lysate; Human liver and Mouse skeletal muscle tissue;. HeLa and HepG2 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185208の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.
特記事項
Flow Cyt (Intra)
1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.

ターゲット情報

  • 機能

    Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.

  • 組織特異性

    Highest expression is found in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas.

  • パスウェイ

    Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.

  • 関連疾患

    Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2) [MIM:239510]. HP-2 is characterized by the accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. The disorder may be causally related to neurologic manifestations, including seizures and mental retardation.

  • 配列類似性

    Belongs to the aldehyde dehydrogenase family.

  • 細胞内局在

    Mitochondrion matrix.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AL4A1_HUMAN antibody
    • aldehyde dehydrogenase 4 antibody
    • aldehyde dehydrogenase 4 family, member A1 antibody
    • Aldehyde dehydrogenase family 4 member A1 antibody
    • Aldehyde dehydrogenase, family 4, subfamily A, member 1 antibody
    • ALDH4 antibody
    • aldh4a1 antibody
    • Delta 1 pyrroline 5 carboxylate dehydrogenase, mitochondrial antibody
    • Delta-1-pyrroline-5-carboxylate dehydrogenase antibody
    • L-glutamate gamma-semialdehyde dehydrogenase antibody
    • mitochondrial antibody
    • mitochondrial delta-1-pyrroline 5-carboxylate dehydrogenase antibody
    • P5C dehydrogenase antibody
    • P5CD antibody
    • P5CDh antibody
    • P5CDhL antibody
    • P5CDhS antibody
    • Pyrroline-5-carboxylate dehydrogenase antibody
    • RP11 128M10.1 antibody
    see all

画像

  • Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
    Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
    Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208) at 1/1000 dilution + Human fetal heart at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 62 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH4A1/P5CDH with ab185208 at 1/250 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
    Immunocytochemistry/ Immunofluorescence - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)

    Immunofluorescent analysis of -20℃ acetone fixed HeLa cells labeling ALDH4A1/P5CDH with ab185208 at 1/250 dilution (red). Secondary antibody: Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi (blue).

  • Flow Cytometry (Intracellular) - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
    Flow Cytometry (Intracellular) - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)

    Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HepG2 cells labeling ALDH4A1/P5CDH with ab185208 at 1/160 dilution (red), or a rabbit IgG isotype control (green), followed by secondary goat anti rabbit IgG (FITC) at 1/150 dilution. 

     

     

  • Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
    Western blot - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
    Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208) at 1/5000 dilution + HepG2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 62 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)

    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling ALDH4A1/P5CDH with ab185208 at 1/250 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)
    Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208)

参考文献 (5)

ab185208 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab185208 は 5 報の論文で使用されています。

  • Lee JS  et al. Overall survival of pancreatic ductal adenocarcinoma is doubled by Aldh7a1 deletion in the KPC mouse. Theranostics 11:3472-3488 (2021). PubMed: 33537098
  • Choi S  et al. Suppression of Foxo3-Gatm by miR-132-3p Accelerates Cyst Formation by Up-Regulating ROS in Autosomal Dominant Polycystic Kidney Disease. Biomol Ther (Seoul) 29:311-320 (2021). PubMed: 33408288
  • Etienne J  et al. Aldehyde dehydrogenases contribute to skeletal muscle homeostasis in healthy, aging, and Duchenne muscular dystrophy patients. J Cachexia Sarcopenia Muscle 11:1047-1069 (2020). PubMed: 32157826
  • Lee JS  et al. Gastric cancer depends on aldehyde dehydrogenase 3A1 for fatty acid oxidation. Sci Rep 9:16313 (2019). PubMed: 31705020
  • Wijermars LGM  et al. Association of Impaired Reactive Aldehyde Metabolism with Delayed Graft Function in Human Kidney Transplantation. Oxid Med Cell Longev 2018:3704129 (2018). PubMed: 30671169

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