Anti-ALDH2 抗体 (ab115198)
Key features and details
- Rabbit polyclonal to ALDH2
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ALDH2 antibody
ALDH2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ALDH2 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide conjugated to KLH, corresponding to amino acids from the N terminal region of ALDH2.
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ポジティブ･コントロール
- IHC-P: Human Liver tissue. Flow Cyt (Intra): HepG2 cells. WB: 293 cell lysate transiently transfected with the ALDH2 gene.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115198の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | (1) |
1/50 - 1/100. Predicted molecular weight: 56 kDa.
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| IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/50 - 1/100. Predicted molecular weight: 56 kDa. |
|
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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パスウェイ
Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2. -
配列類似性
Belongs to the aldehyde dehydrogenase family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 217 Human
- Omim: 100650 Human
- SwissProt: P05091 Human
- Unigene: 604551 Human
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別名
- Acetaldehyde dehydrogenase 2 antibody
- Aldehyde dehydrogenase 2 family (mitochondrial) antibody
- Aldehyde dehydrogenase 2 family antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody (ab115198)ab115198 at 10 µg/ml staining ALDH2 Formalin-fixed, Paraffin-embedded Human Liver tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Flow Cytometry (Intracellular) - Anti-ALDH2 antibody (ab115198)Intracellular flow cytometric analysis of HepG2 cells, staining ALDH2 with ab115198 (bottom histogram), compared to a negative control cell (top histogram). A FITC-conjugated goat-anti-rabbit IgG was used as the secondary antibody.
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Western blot - Anti-ALDH2 antibody (ab115198)All lanes : Anti-ALDH2 antibody (ab115198) at 1/50 dilution
Lane 1 : 293 cell lysate, non-transfected
Lane 2 : 293 cell lysate, transiently transfected with ALDH2 gene
Lysates/proteins at 2 µg per lane.
Predicted band size: 56 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab115198 は論文での使用が確認できていません。