Anti-ALDH1L1 抗体 [EPR12744(B)] (ab175198)
![Western blot - Anti-ALDH1L1 antibody [EPR12744(B)] (ab175198)](https://www.abcam.co.jp/ps/products/175/ab175198/Images/ab175198-201551-anti-aldh1l1-antibody-epr12744b-western-blot.jpg "Western blot - Anti-ALDH1L1 antibody [EPR12744(B)] (ab175198)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12744(B)] to ALDH1L1
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ALDH1L1 antibody [EPR12744(B)]
ALDH1L1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12744(B)] to ALDH1L1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details
適用なし: ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human fetal liver and kidney lysates; Human kidney and liver tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12744(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175198の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/1000 - 1/5000. Predicted molecular weight: 99 kDa.
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| IP |
1/10 - 1/100.
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| 特記事項 |
|---|
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 99 kDa. |
|
IP
1/10 - 1/100. |
ターゲット情報
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組織特異性
Highly expressed in liver, pancreas and kidney. -
配列類似性
In the N-terminal section; belongs to the GART family.
In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.
Contains 1 acyl carrier domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 10840 Human
- Entrez Gene: 107747 Mouse
- Entrez Gene: 64392 Rat
- Omim: 600249 Human
- SwissProt: O75891 Human
- SwissProt: Q8R0Y6 Mouse
- SwissProt: P28037 Rat
- Unigene: 434435 Human
see all -
別名
- 10 formyltetrahydrofolate dehydrogenase antibody
- 10 FTHFDH antibody
- 10-formyltetrahydrofolate dehydrogenase antibody
see all
画像
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Western blot - Anti-ALDH1L1 antibody [EPR12744(B)] (ab175198)All lanes : Anti-ALDH1L1 antibody [EPR12744(B)] (ab175198) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 99 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12744(B)] (ab175198)](https://www.abcam.co.jp/ps/products/175/ab175198/Images/ab175198-201550-anti-aldh1l1-antibody-epr12744b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12744(B)] (ab175198)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ALDH1L1 with ab175198 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12744(B)] (ab175198)](https://www.abcam.co.jp/ps/products/175/ab175198/Images/ab175198-201549-anti-aldh1l1-antibody-epr12744b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12744(B)] (ab175198)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH1L1 with ab175198 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-ALDH1L1 antibody [EPR12744(B)] (ab175198)](https://www.abcam.co.jp/ps/products/175/ab175198/Images/ab175198-201548-anti-aldh1l1-antibody-epr12744b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ALDH1L1 antibody [EPR12744(B)] (ab175198)Western blot analysis on immunoprecipitation pellet from Human fetal liver lysate labeling ALDH1L1 using ab175198 at a 1/10 dilution.
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![Anti-ALDH1L1 antibody [EPR12744(B)] (ab175198)](https://www.abcam.co.jp/ps/products/175/ab175198/Images/ab175198-5-benefits-of-recombinant-antibodies.png)
Anti-ALDH1L1 antibody [EPR12744(B)] (ab175198)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab175198 は 5 報の論文で使用されています。
- Lee JS et al. Overall survival of pancreatic ductal adenocarcinoma is doubled by Aldh7a1 deletion in the KPC mouse. Theranostics 11:3472-3488 (2021). PubMed: 33537098
- Krupenko SA & Sharma J Is ALDH1L1 Elevated in Lung Cancer? Comment on: Lee, S.-H.; et al. "The Combination of Loss of ALDH1L1 Function and Phenformin Treatment Decreases Tumor Growth in KRAS-Driven Lung Cancer" Cancers 2020, 12, 1382. Cancers (Basel) 13:N/A (2021). PubMed: 33918472
- Lee SH et al. The Combination of Loss of ALDH1L1 Function and Phenformin Treatment Decreases Tumor Growth in KRAS-Driven Lung Cancer. Cancers (Basel) 12:N/A (2020). PubMed: 32481524
- Lee JS et al. Gastric cancer depends on aldehyde dehydrogenase 3A1 for fatty acid oxidation. Sci Rep 9:16313 (2019). PubMed: 31705020
- Kang JH et al. Aldehyde dehydrogenase inhibition combined with phenformin treatment reversed NSCLC through ATP depletion. Oncotarget 7:49397-49410 (2016). PubMed: 27384481