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Neuroscience Cell Type Marker Glia marker Astrocyte marker
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Anti-ALDH1L1 抗体 - Astrocyte Marker (ab87117)

  • Datasheet
  • SDS
Reviews (11)Q&A (4)References (74)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (ab87117)

    Key features and details

    • Rabbit polyclonal to ALDH1L1 - Astrocyte Marker
    • Suitable for: WB
    • Reacts with: Mouse, Rat
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-ALDH1L1 antibody - Astrocyte Marker
      ALDH1L1 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to ALDH1L1 - Astrocyte Marker
    • 由来種

      Rabbit
    • アプリケーション

      適用あり: WBmore details
    • 種交差性

      交差種: Mouse, Rat
      交差が予測される動物種: Orangutan
    • 免疫原

      Synthetic peptide within Mouse ALDH1L1 aa 300-400 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
      Database link: Q8R0Y6
      (Peptide available as ab103935)

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 1% BSA, 98.98% PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Neuroscience
      • Cell Type Marker
      • Glia marker
      • Astrocyte marker
      • Cell Biology
      • Apoptosis
      • Intracellular
      • Caspases etc
      • Caspases
      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Cancer
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      • p53 pathway
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      • Metabolism
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      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism
      • Metabolism
      • Types of disease
      • Cancer
      • Neuroscience
      • Development

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab87117の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (2)
    Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 99 kDa).
    特記事項
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 99 kDa).

    ターゲット情報

    • 組織特異性

      Highly expressed in liver, pancreas and kidney.
    • 配列類似性

      In the N-terminal section; belongs to the GART family.
      In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.
      Contains 1 acyl carrier domain.
    • 細胞内局在

      Cytoplasm.
    • Target information above from: UniProt accession O75891 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 107747 Mouse
      • Entrez Gene: 64392 Rat
      • SwissProt: Q8R0Y6 Mouse
      • SwissProt: Q5RFM9 Orangutan
      • SwissProt: P28037 Rat
      • Unigene: 30035 Mouse
      • Unigene: 2328 Rat
      • 別名

        • 10 formyltetrahydrofolate dehydrogenase antibody
        • 10 FTHFDH antibody
        • 10-formyltetrahydrofolate dehydrogenase antibody
        • 10-FTHFDH antibody
        • AL1L1_HUMAN antibody
        • Aldehyde dehydrogenase 1 family member L1 antibody
        • Aldehyde dehydrogenase family 1 member L1 antibody
        • aldh1l1 antibody
        • Cytosolic 10-formyltetrahydrofolate dehydrogenase antibody
        • DKFZp781N0997 antibody
        • EC 1.5.1.6 antibody
        • FDH antibody
        • formyltetrahydrofolate dehydrogenase antibody
        • FTHFD antibody
        • OTTHUMP00000155221 antibody
        • OTTHUMP00000214696 antibody
        • OTTHUMP00000214700 antibody
        • OTTHUMP00000222724 antibody
        see all

      画像

      • Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (ab87117)
        Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (ab87117)
        All lanes : Anti-ALDH1L1 antibody - Astrocyte Marker (ab87117) at 1 µg/ml

        Lane 1 : Liver (Mouse) Tissue Lysate
        Lane 2 : Brain (Mouse) Tissue Lysate
        Lane 3 : Brain (Rat) Tissue Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 99 kDa
        Observed band size: 100 kDa why is the actual band size different from the predicted?


        Exposure time: 4 minutes

      プロトコール

      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      データシートおよび資料

      • SDS download

      • Datasheet download

        Download

      参考文献 (74)

      ab87117 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab87117 は 74 報の論文で使用されています。

      • Endo M  et al. Wnt5a/Ror2 promotes Nrf2-mediated tissue protective function of astrocytes after brain injury. Glia 72:411-432 (2024). PubMed: 37904612
      • Kurabayashi N  et al. Neocortical neuronal production and maturation defects in the TcMAC21 mouse model of Down syndrome. iScience 26:108379 (2023). PubMed: 38025769
      • Gleichman AJ  et al. A toolbox of astrocyte-specific, serotype-independent adeno-associated viral vectors using microRNA targeting sequences. Nat Commun 14:7426 (2023). PubMed: 37973910
      • Reyes-Ortiz AM  et al. Single-nuclei transcriptome analysis of Huntington disease iPSC and mouse astrocytes implicates maturation and functional deficits. iScience 26:105732 (2023). PubMed: 36590162
      • Itoh N  et al. Estrogen receptor beta in astrocytes modulates cognitive function in mid-age female mice. Nat Commun 14:6044 (2023). PubMed: 37758709
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-10 of 15 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ALDH1L1 antibody - Astrocyte Marker

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (brain)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris/EDTA
      Permeabilization
      No
      Specification
      brain
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Apr 05 2022

      Western blot abreview for Anti-ALDH1L1 antibody - Astrocyte Marker

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (spinal cord)
      Gel Running Conditions
      Reduced Denaturing (8%)
      Loading amount
      20 µg
      Specification
      spinal cord
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Yu Chen

      Verified customer

      投稿 Mar 15 2021

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ALDH1L1 antibody - Astrocyte Marker

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Brain)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
      Permeabilization
      No
      Specification
      Brain
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Jan 11 2021

      Immunohistochemistry (Frozen sections) abreview for Anti-ALDH1L1 antibody - Astrocyte Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Brain)
      Permeabilization
      No
      Specification
      Brain
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Fixative
      Acetone
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Jan 11 2021

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ALDH1L1 antibody - Astrocyte Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Brain)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
      Permeabilization
      No
      Specification
      Brain
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Jan 11 2021

      Western blot abreview for Anti-ALDH1L1 antibody - Astrocyte Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Brain lysate)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      25 µg
      Specification
      Brain lysate
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Jun 08 2018

      Immunohistochemistry (Frozen sections) abreview for Anti-ALDH1L1 antibody - Astrocyte Marker

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Sample
      Mouse Tissue sections (brain-astrocytes)
      Specification
      brain-astrocytes
      Permeabilization
      No
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Jun 05 2014

      Immunocytochemistry abreview for Anti-ALDH1L1 antibody - Astrocyte Marker

      Good
      Abreviews
      Abreviews
      Application
      Immunocytochemistry
      Sample
      Mouse Cultured Cells (mouse cortex)
      Specification
      mouse cortex
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.5% triton x100
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 May 29 2012

      Question

      Yes, I would like to test the ab87117 on human tissue. I look forward to getting the discount code.

      Read More

      Abcam community

      Verified customer

      Asked on Sep 18 2012

      Answer

      I am very pleased to hear you would like to accept our offer and test ab87117 in human samples.

      This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for human samples and include this code in the Additional Comments section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

      For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews

      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

      1) Call to place your order and mention the code to our customer service department;
      2) Include the code in your fax order;
      3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us.

      We look forward to receiving your Abreview and wish you luck with your research.
      The terms and conditions applicable to this offer can be found here:

      https://www.abcam.com/collaborationdiscount

      Read More

      Abcam Scientific Support

      Answered on Sep 18 2012

      Question

      Thanks for your reply. Unfortunately, GFAP isn't a good protoplasmic astrocyte marker, and I was hoping to use AldH1L1 instead.

      Could you please clarify: are you saying that your ab87117 AldH1L1 antibody is not suitable for immunostaining human brain paraffin sections?

      Thanks,

      Read More

      Abcam community

      Verified customer

      Asked on Sep 17 2012

      Answer

      Thank you for your reply.

      ab87117 is suitable for paraffin-embedded sections but it hasnt been tested on human samples yet. Therefore, I can offer a discount off a future purchase if you buy ab87117 now, test it in human and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know that you would like to proceed and test ab87117 in human. I will then send a discount code. This code must be issued before purchasing ab87117 so please wait for my reply before ordering.

      2. Purchase ab87117 either by phone, fax, or online (www.abcam.com).

      3. Test it in human.

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
      Please remember that submission of the Abreview is sufficient for the discount code to become active.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab87117 turns out to be unsuitable for human, you will still receive the discount on your next purchase after your Abreview has been submitted.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Sep 17 2012

      1-10 of 15 Abreviews or Q&A

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      For licensing inquiries, please contact partnerships@abcam.com

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