AB227964

Anti-ALDH1A1 抗体

Anti-ALDH1A1 antibody

JP Deleterious:医薬用外劇物

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(9 Publications)

Rabbit Polyclonal ALDH1A1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Zebrafish, Human, Mouse, Rat samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH1A1.

別名を表示する

ALDC, ALDH1, PUMB1, ALDH1A1, Aldehyde dehydrogenase 1A1, 3-deoxyglucosone dehydrogenase, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Retinal dehydrogenase 1, RALDH 1, RalDH1

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-ALDH1A1 antibody (AB227964)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ALDH1A1 antibody (AB227964)

Methanol-fixed A549 (human lung carcinoma cell line) cells stained for ALDH1A1 (green) using ab227964 at 1/500 dilution in ICC/IF.

Nuclear counter stain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (AB227964)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (AB227964)

Paraffin-embedded human gastric cancer tissue stained for ALDH1A1 using ab227964 at 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : EDTA based, pH 8.0 buffer, 15 mins.

Supplier Data

Western blot - Anti-ALDH1A1 antibody (AB227964)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-ALDH1A1 antibody (ab227964) at 1/10000 dilution

All lanes:

A549 (human lung carcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 54 kDa

false

Supplier Data

Western blot - Anti-ALDH1A1 antibody (AB227964)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ALDH1A1 antibody (ab227964) at 1/10000 dilution

Lane 1:

A549 whole cell extracts at 30 µg

Lane 2:

H1299 whole cell extracts at 30 µg

Lane 3:

HCT116 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 55 kDa

false

Supplier Data

Western blot - Anti-ALDH1A1 antibody (AB227964)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ALDH1A1 antibody (ab227964) at 1/10000 dilution

All lanes:

PG-4 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 55 kDa

false

Supplier Data

Western blot - Anti-ALDH1A1 antibody (AB227964)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-ALDH1A1 antibody (ab227964) at 1/5000 dilution

All lanes:

Mouse liver lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 54 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (AB227964)

Paraffin-embedded whole-mount zebrafish embryos tissue stained for Aldh1a2 protein with ab227964 at a 1/200 dilution in immunohistochemical analysis.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human, Zebrafish

アプリケーション

WB, ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human ALDH1A1. The exact immunogen used to generate this antibody is proprietary information.

P00352

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "Methanol fixed." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ALDH1A1 also known as aldehyde dehydrogenase 1 family member A1 is an enzyme with a molecular weight of approximately 54 kDa. It functions by catalyzing the irreversible oxidation of aldehydes to carboxylic acids which is an important step in detoxifying alcohol-derived acetaldehyde and other aldehydes. ALDH1A1 is expressed in various tissues including liver kidney and cornea. Its activity allows for maintaining cellular homeostasis by processing endogenous and exogenous aldehydes.

Biological function summary

ALDH1A1 plays significant roles in the biosynthesis of retinoic acid an important regulator of gene expression in developmental and adult processes. It does not form part of a larger enzyme complex but functions independently. The ALDH1A1 proteins contribute to the regulation of cell proliferation differentiation and survival. Researchers often measure its expression using methods like the ALDH1A1 ELISA which quantifies the protein levels in biological samples.

Pathways

The ALDH1A1 enzyme is notably implicated in the retinoid metabolism pathway and the detoxification of alcohol-derived aldehydes within the body. It is closely related to other members of the aldehyde dehydrogenase family including ALDH2 which shares similar detoxification functions. ALDH1A1’s role in retinoic acid production links it to gene expression pathways associated with cell differentiation and growth.

ALDH1A1 has associations with cancer and alcohol-induced liver disease. Its elevated expression in cancerous tissues often correlates with stem-cell like characteristics and chemoresistance. A connection exists between ALDH1A1 and ALDH2 in the context of alcohol metabolism where ALDH1A1's role in detoxifying acetaldehyde contributes to the lower risk of alcohol-induced damage. Studies explore ALDH1A1 antibodies and inhibitors as potential therapeutic approaches for these conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Cytosolic dehydrogenase that catalyzes the irreversible oxidation of a wide range of aldehydes to their corresponding carboxylic acid (PubMed : 12941160, PubMed : 15623782, PubMed : 17175089, PubMed : 19296407, PubMed : 25450233, PubMed : 26373694). Functions downstream of retinol dehydrogenases and catalyzes the oxidation of retinaldehyde into retinoic acid, the second step in the oxidation of retinol/vitamin A into retinoic acid (By similarity). This pathway is crucial to control the levels of retinol and retinoic acid, two important molecules which excess can be teratogenic and cytotoxic (By similarity). Also oxidizes aldehydes resulting from lipid peroxidation like (E)-4-hydroxynon-2-enal/HNE, malonaldehyde and hexanal that form protein adducts and are highly cytotoxic. By participating for instance to the clearance of (E)-4-hydroxynon-2-enal/HNE in the lens epithelium prevents the formation of HNE-protein adducts and lens opacification (PubMed : 12941160, PubMed : 15623782, PubMed : 19296407). Functions also downstream of fructosamine-3-kinase in the fructosamine degradation pathway by catalyzing the oxidation of 3-deoxyglucosone, the carbohydrate product of fructosamine 3-phosphate decomposition, which is itself a potent glycating agent that may react with lysine and arginine side-chains of proteins (PubMed : 17175089). Has also an aminobutyraldehyde dehydrogenase activity and is probably part of an alternative pathway for the biosynthesis of GABA/4-aminobutanoate in midbrain, thereby playing a role in GABAergic synaptic transmission (By similarity).

See full target information ALDH1A1

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文献 (9)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer therapeutics 24:453-463 PubMed39676352

2024

All-trans Retinoic Acid Sensitizes Epithelial Ovarian Cancer to PARP Inhibition after Exposure to Cisplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Bingjie Mei,Junyang Li,Dengfeng Wang,Lu Feng,Jianming Huang,Guonan Zhang

BMC cancer 24:1370 PubMed39516821

2024

NSDHL contributes to breast cancer stem-like cell maintenance and tumor-initiating capacity through TGF-β/Smad signaling pathway in MCF-7 tumor spheroid.

Applications

Unspecified application

Species

Unspecified reactive species

So-Hyun Yoon,Sangeun Lee,Hoe Suk Kim,Junhyuk Song,Moonjou Baek,Seungyeon Ryu,Han-Byoel Lee,Hyeong-Gon Moon,Dong-Young Noh,Sangyong Jon,Wonshik Han

Analytical cellular pathology (Amsterdam) 2022:5632164 PubMed36247876

2022

TAZ Regulates the Cisplatin Resistance of Epithelial Ovarian Cancer Cells via the ANGPTL4/SOX2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Caihong Li,Qin Wang,Youzhen Luo,Juan Xiang

Journal of cellular and molecular medicine 24:8545-8556 PubMed32596945

2020

A SNP of miR-146a is involved in bladder cancer relapse by affecting the function of bladder cancer stem cells via the miR-146a signallings.

Applications

Unspecified application

Species

Unspecified reactive species

Tianen Wang,Yanfeng Yang,Zhiyong Wang,Xuechong Zhang,Dongsheng Li,Jinxing Wei

Oncology letters 20:1752-1760 PubMed32724418

2020

Association between APOBEC3s and HPV16 E2 gene hypermutation in Uygur females with cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Sui,Zhen Jiao,Hongxiang Chen,Mayinuer Niyazi,Lin Wang

Frontiers in endocrinology 10:623 PubMed31620083

2019

Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells.

Applications

ICC/IF

Species

Rat

Patrick A Fletcher,Kosara Smiljanic,Rafael Maso Prévide,James R Iben,Tianwei Li,Milos B Rokic,Arthur Sherman,Steven L Coon,Stanko S Stojilkovic

Oncogene 38:4047-4060 PubMed30705400

2019

WNT signaling modulates PD-L1 expression in the stem cell compartment of triple-negative breast cancer.

Applications

Unspecified application

Species

Human

Lorenzo Castagnoli,Valeria Cancila,Sandra L Cordoba-Romero,Simona Faraci,Giovanna Talarico,Beatrice Belmonte,Marilena V Iorio,Matteo Milani,Tatiana Volpari,Claudia Chiodoni,Alfredo Hidalgo-Miranda,Elda Tagliabue,Claudio Tripodo,Sabina Sangaletti,Massimo Di Nicola,Serenella M Pupa

Journal of cellular and molecular medicine 22:1337-1349 PubMed29193723

2017

TRAF6 regulates tumour metastasis through EMT and CSC phenotypes in head and neck squamous cell carcinoma.

Applications

Species

Human

Lei Chen,Yi-Cun Li,Lei Wu,Guang-Tao Yu,Wen-Feng Zhang,Cong-Fa Huang,Zhi-Jun Sun

Journal of experimental & clinical cancer research 36:116 PubMed28865486

2017

NLRP3 inflammasome activation promotes inflammation-induced carcinogenesis in head and neck squamous cell carcinoma.

Applications

WB, ICC/IF, IHC-P

Species

Mouse, Mouse, Mouse

Cong-Fa Huang,Lei Chen,Yi-Cun Li,Lei Wu,Guang-Tao Yu,Wen-Feng Zhang,Zhi-Jun Sun

View all publications

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Anti-ALDH1A1 抗体

Anti-ALDH1A1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ALDH1A1 antibody (AB227964)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (AB227964)

Western blot - Anti-ALDH1A1 antibody (AB227964)

Western blot - Anti-ALDH1A1 antibody (AB227964)

Western blot - Anti-ALDH1A1 antibody (AB227964)

Western blot - Anti-ALDH1A1 antibody (AB227964)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (AB227964)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (9)

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