JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB222923

Anti-Albumin 抗体 [EPR20195] - Low endotoxin, Azide free

Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Recombinant Monoclonal Albumin antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

別名を表示する

GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, ALB, Albumin

3 Images
Flow Cytometry (Intracellular) - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (AB222923)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (AB222923)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Albumin with ab207327 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207327).

Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (AB222923)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (AB222923)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) or HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Albumin with ab207327 at 1/500 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on HepG2 cell line.

Negative control : HeLa (PMID : 10476216 and 8314088)).

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA glycerol and sodium azide (ab207327).

Immunoprecipitation - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (AB222923)
  • IP

Supplier Data

Immunoprecipitation - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (AB222923)

Albumin was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab207327 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab207327 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HepG2 whole cell lysate, 10μg (Input).

Lane 2 : ab207327 IP in HepG2 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab207327 in HepG2 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207327).

All lanes:

Immunoprecipitation - Anti-Albumin antibody [EPR20195] (<a href='/products/primary-antibodies/albumin-antibody-epr20195-ab207327'>ab207327</a>)

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

関連する標識済み抗体及び組成の異なる製品 (4)

  • Carrier free

    Anti-Albumin antibody [EPR20195] - BSA and Azide free

  • Unconjugated

    Anti-Albumin antibody [EPR20195]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Albumin antibody [EPR20195]

  • HRP

    HRP Anti-Albumin antibody [EPR20195]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20195

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse, Rat, Human

アプリケーション

Flow Cyt (Intra), IP, WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p>Permeabilization is required.</p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

製品の詳細

ab222923 is the carrier-free version of ab207327.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed : 19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed : 6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed : 6234017).
See full target information ALB
style
left-column

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 12:3281-3297 PubMed35967275

2022

SYNCRIP controls miR-137 and striatal learning in animal models of methamphetamine abstinence.

Applications

Unspecified application

Species

Unspecified reactive species

Baeksun Kim,Sung Hyun Tag,Eunjoo Nam,Suji Ham,Sujin Ahn,Juhwan Kim,Doo-Wan Cho,Sangjoon Lee,Young-Su Yang,Seung Eun Lee,Yong Sik Kim,Il-Joo Cho,Kwang Pyo Kim,Su-Cheol Han,Heh-In Im

Cell death discovery 8:77 PubMed35194023

2022

Long noncoding RNA SNHG1 silencing accelerates hepatocyte-like cell differentiation of bone marrow-derived mesenchymal stem cells to alleviate cirrhosis via the microRNA-15a/SMURF1/UVRAG axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Sun,Xuedong Sun,Sean Hu,Maoqiang Wang,Na Ma,Junhui Chen,Feng Duan

The Journal of biological chemistry 292:2773-2784 PubMed28049731

2017

Essential Role for Polycomb Group Protein Pcgf6 in Embryonic Stem Cell Maintenance and a Noncanonical Polycomb Repressive Complex 1 (PRC1) Integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Wukui Zhao,Huan Tong,Yikai Huang,Yun Yan,Huajian Teng,Yin Xia,Qing Jiang,Jinzhong Qin
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB106582

Anti-Albumin antibody

Lab Essentials

Western blot - Anti-Albumin antibody (AB106582)

  • 4
  • 11
Primary Antibodies

AB174658

Biotin Anti-Albumin antibody

  • 0
  • 0
Primary Antibodies

AB112980

Anti-Albumin antibody

Lab Essentials

  • 0
  • 0
Primary Antibodies

AB137885

Anti-Albumin antibody [EPSISR1]

20ul selling size|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-Albumin antibody [EPSISR1] (AB137885)

  • 0
  • 0
Primary Antibodies

AB175934

Anti-Albumin antibody [EPR12780(B)]

RabMAb|Recombinant|Lab Essentials

Western blot - Anti-Albumin antibody [EPR12780(B)] (AB175934)

  • 0
  • 0
Primary Antibodies

AB151742

Anti-Albumin antibody [EPSISR3]

20ul selling size|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-Albumin antibody [EPSISR3] (AB151742)

  • 0
  • 0
Primary Antibodies

AB240109

Anti-Albumin antibody [EPSISR1] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Albumin antibody [EPSISR1] - BSA and Azide free (AB240109)

  • 0
  • 0
Primary Antibodies

AB236492

Anti-Albumin antibody [ALB/2144]

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody [ALB/2144] (AB236492)

  • 0
  • 0
Primary Antibodies

AB112976

HRP Anti-Albumin antibody

  • 0
  • 0

組合せ製品

Assay Kits

AB235628

Albumin (BCG) Assay Kit (Colorimetric)

Functional Studies - Albumin (BCG) Assay Kit (Colorimetric) (AB235628)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB125011

Anti-TSG101 antibody [EPR7130(B)]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Anti-TSG101 antibody [EPR7130(B)] (AB125011)

  • 4
  • 10
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com