AB175934

Anti-Albumin 抗体 [EPR12780(B)]

Anti-Albumin antibody [EPR12780(B)]

RabMAb
Recombinant
Lab Essentials
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(4 Publications)

Rabbit Recombinant Monoclonal Albumin antibody. Suitable for WB and reacts with Cow samples. Cited in 4 publications.

別名を表示する

Albumin, BSA, ALB

1 Images

Lab

Western blot - Anti-Albumin antibody [EPR12780(B)] (AB175934)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Albumin antibody [EPR12780(B)] (ab175934) at 1/1000 dilution

All lanes:

Bovine serum albumin lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR12780(B)

アイソタイプ

IgG

キャリアフリー

交差種

Cow

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Cow": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.

Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (Probable). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (PubMed : 22677715). The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (Probable). The rank order of affinity is zinc > calcium > magnesium (Probable). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed : 6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed : 6234017).

See full target information Albumin

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 133:e70052 PubMed40671091

2025

Downregulation of lncRNA H19 Enhances Radiosensitivity in Colorectal Cancer by Inactivation of the ATR/CHK1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yuting Liang,Wanlu Zhang,Ao Dungerile,Juping Wang,Runmei Wang

Experimental & molecular medicine 56:583-599 PubMed38424192

2024

Mechanical overload-induced release of extracellular mitochondrial particles from tendon cells leads to inflammation in tendinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ziming Chen,Mengyuan Li,Peilin Chen,Andrew Tai,Jiayue Li,Euphemie Landao Bassonga,Junjie Gao,Delin Liu,David Wood,Brendan F Kennedy,Qiujian Zheng,Ming H Zheng

Journal of extracellular vesicles 11:e12266 PubMed36124834

2022

Comparative analysis of tangential flow filtration and ultracentrifugation, both combined with subsequent size exclusion chromatography, for the isolation of small extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Kekoolani S Visan,Richard J Lobb,Sunyoung Ham,Luize G Lima,Carlos Palma,Chai Pei Zhi Edna,Li-Ying Wu,Harsha Gowda,Keshava K Datta,Gunter Hartel,Carlos Salomon,Andreas Möller

Journal of extracellular vesicles 10:e12087 PubMed33936570

2021

Quantitative proteomic analysis of extracellular vesicle subgroups isolated by an optimized method combining polymer-based precipitation and size exclusion chromatography.

Applications

Unspecified application

Species

Unspecified reactive species

Juan A Martínez-Greene,Karina Hernández-Ortega,Ricardo Quiroz-Baez,Osbaldo Resendis-Antonio,Israel Pichardo-Casas,David A Sinclair,Bogdan Budnik,Alfredo Hidalgo-Miranda,Eileen Uribe-Querol,María Del Pilar Ramos-Godínez,Eduardo Martínez-Martínez

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Albumin 抗体 [EPR12780(B)]

Anti-Albumin antibody [EPR12780(B)]

Western blot - Anti-Albumin antibody [EPR12780(B)] (AB175934)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

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Abcam product promise