Anti-Albumin antibody (ab106582) is a chicken polyclonal antibody detecting Albumin in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.
- Over 50 publications
別名を表示する
GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, ALB, Albumin
- WB
Supplier Data
Western blot - Anti-Albumin antibody (AB106582)
1h incubation at RT in 5% NFDM/TBST.
All lanes:
Western blot - Anti-Albumin antibody (ab106582) at 1 µg/mL
All lanes:
Mouse liver tissue lysate at 10 µg
Secondary
All lanes:
Goat anti- chicken IgY HRP conjugate at 1/10000 dilution
false
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling albumin with ab106582 at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)
Immunohistochemical analysis of paraffin embedded Mouse liver tissue section labelling Albumin with ab106582 at 5 μg/ml.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling albumin with ab106582 at 10 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling albumin with ab106582 at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)
Immunohistochemical analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling albumin with ab106582 at 20 µg/mL, followed by goat anti-chicken IgY secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
- WB
Supplier Data
Western blot - Anti-Albumin antibody (AB106582)
1h incubation at RT in 5% NFDM/TBST.
All lanes:
Western blot - Anti-Albumin antibody (ab106582) at 1 µg/mL
All lanes:
Human liver tissue lysate at 10 µg
Secondary
All lanes:
Goat anti- chicken IgY HRP conjugate at 1/10000 dilution
false
- WB
Supplier Data
Western blot - Anti-Albumin antibody (AB106582)
1h incubation at RT in 5% NFDM/TBST.
All lanes:
Western blot - Anti-Albumin antibody (ab106582) at 1 µg/mL
All lanes:
Rat liver tissue lysate at 10 µg
Secondary
All lanes:
Goat anti- chicken IgY HRP conjugate at 1/10000 dilution
false
- WB
CiteAb
Western blot - Anti-Albumin antibody (AB106582)
Albumin western blotting using Anti-Albumin antibody ab106582. Publication image and figure legend from Tan, Z., Chen, L., et al., 2020, Ann Clin Transl Neurol, PubMed 32302063.
The effect of FK866 on brain edema, and BBB disruption at 1 day after TBI or sham operation. (A) Quantification of brain water content. The bar represents the mean ± SD. n = 6. The bar represents mean ± SD, **p < < 0.01 versus sham, ##p < < 0.01 versus vehicle control. (B) Quantification of Evans blue dye extravasation. The bar represents the mean ± SD. n = 6. **p < < 0.01 versus sham. ##p < < 0.01 versus vehicle control. (C) Representative western blots and Densitometric quantification of albumin. The bar represents the mean ± SD. N = 6. **p < < 0.01 versus sham group; ##p < < 0.01 versus TBI + vehicle group. (D) Representative western blots and densitometric quantification of occluding. The bar represents the mean ± SD. n = 6. **p < < 0.01 versus sham group; ##p < < 0.01 versus TBI + vehicle group.
false
Reactivity data
製品の詳細
Antibodies should not be exposed to prolonged high temperatures.
Product Specifications
Anti-Albumin antibody (ab106582) is a chicken polyclonal antibody and is validated for use in IF, IHC-P, WB in human, mouse, rat samples.
Anti-Albumin antibody (ab106582) specifically detects Albumin (UniProt ID: P02768; Molecular weight: 66kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Albumin antibody (ab106582) has high sensitivity and specificity.
Anti-Albumin antibody (ab106582) has been cited over 52 times in peer reviewed journals and is trusted by the scientific community.
Anti-Albumin antibody (ab106582) has 11 independent reviews from customers.
出荷温度及び保存条件
製品の状態
精製方法
バッファー組成
出荷温度
短期保存温度
長期保存温度
補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.
Pathways
More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.
製品プロトコール
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文献 (67)
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