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AB106582

Anti-Albumin 抗体

Anti-Albumin antibody

4

(11 Reviews)

|

(67 Publications)

Anti-Albumin antibody (ab106582) is a chicken polyclonal antibody detecting Albumin in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.

- Over 50 publications

別名を表示する

GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, ALB, Albumin

9 Images
Western blot - Anti-Albumin antibody (AB106582)
  • WB

Supplier Data

Western blot - Anti-Albumin antibody (AB106582)

1h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-Albumin antibody (ab106582) at 1 µg/mL

All lanes:

Mouse liver tissue lysate at 10 µg

Secondary

All lanes:

Goat anti- chicken IgY HRP conjugate at 1/10000 dilution

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling albumin with ab106582 at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)

Immunohistochemical analysis of paraffin embedded Mouse liver tissue section labelling Albumin with ab106582 at 5 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling albumin with ab106582 at 10 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling albumin with ab106582 at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody (AB106582)

Immunohistochemical analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling albumin with ab106582 at 20 µg/mL, followed by goat anti-chicken IgY secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Western blot - Anti-Albumin antibody (AB106582)
  • WB

Supplier Data

Western blot - Anti-Albumin antibody (AB106582)

1h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-Albumin antibody (ab106582) at 1 µg/mL

All lanes:

Human liver tissue lysate at 10 µg

Secondary

All lanes:

Goat anti- chicken IgY HRP conjugate at 1/10000 dilution

false

Western blot - Anti-Albumin antibody (AB106582)
  • WB

Supplier Data

Western blot - Anti-Albumin antibody (AB106582)

1h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-Albumin antibody (ab106582) at 1 µg/mL

All lanes:

Rat liver tissue lysate at 10 µg

Secondary

All lanes:

Goat anti- chicken IgY HRP conjugate at 1/10000 dilution

false

Western blot - Anti-Albumin antibody (AB106582)
  • WB

CiteAb

Western blot - Anti-Albumin antibody (AB106582)

Albumin western blotting using Anti-Albumin antibody ab106582. Publication image and figure legend from Tan, Z., Chen, L., et al., 2020, Ann Clin Transl Neurol, PubMed 32302063.

The effect of FK866 on brain edema, and BBB disruption at 1 day after TBI or sham operation. (A) Quantification of brain water content. The bar represents the mean ± SD. n = 6. The bar represents mean ± SD, **p < < 0.01 versus sham, ##p < < 0.01 versus vehicle control. (B) Quantification of Evans blue dye extravasation. The bar represents the mean ± SD. n = 6. **p < < 0.01 versus sham. ##p < < 0.01 versus vehicle control. (C) Representative western blots and Densitometric quantification of albumin. The bar represents the mean ± SD. N = 6. **p < < 0.01 versus sham group; ##p < < 0.01 versus TBI + vehicle group. (D) Representative western blots and densitometric quantification of occluding. The bar represents the mean ± SD. n = 6. **p < < 0.01 versus sham group; ##p < < 0.01 versus TBI + vehicle group.

false

Key facts

宿主種

Chicken

クローン性

Polyclonal

アイソタイプ

IgY

キャリアフリー

No

交差種

Mouse, Human, Rat

アプリケーション

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human ALB. The exact immunogen used to generate this antibody is proprietary information.

P02768

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-20 µg/mL", "IHCP-species-notes": "<p>IHC-P/IF: 10-20 μg/mL; IHC-P: 2-10 μg/mL.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-20 µg/mL", "IHCP-species-notes": "<p>IHC-P/IF: 10-20 μg/mL; IHC-P: 2-10 μg/mL.</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-20 µg/mL", "IHCP-species-notes": "<p>IHC-P/IF: 10-20 μg/mL; IHC-P: 2-10 μg/mL.</p>" } } }

製品の詳細

Antibodies should not be exposed to prolonged high temperatures.

Product Specifications
Anti-Albumin antibody (ab106582) is a chicken polyclonal antibody and is validated for use in IF, IHC-P, WB in human, mouse, rat samples.
Anti-Albumin antibody (ab106582) specifically detects Albumin (UniProt ID: P02768; Molecular weight: 66kDa) and is sold in 100 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-Albumin antibody (ab106582) has high sensitivity and specificity.
Anti-Albumin antibody (ab106582) has been cited over 52 times in peer reviewed journals and is trusted by the scientific community.
Anti-Albumin antibody (ab106582) has 11 independent reviews from customers.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed : 19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed : 6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed : 6234017).
See full target information ALB

文献 (67)

Recent publications for all applications. Explore the full list and refine your search

Aging 17:1929-1965 PubMed40748658

2025

Exosomes released from senescent cells and circulatory exosomes isolated from human plasma reveal aging-associated proteomic and lipid signatures.

Applications

Unspecified application

Species

Unspecified reactive species

Sandip Kumar Patel,Joanna Bons,Jacob P Rose,Jessie R Chappel,Rebecca L Beres,Mark A Watson,Corey Webster,Jordan B Burton,Roland Bruderer,Pierre-Yves Desprez,Lukas Reiter,Judith Campisi,Erin S Baker,Birgit Schilling

Biology 14: PubMed40563838

2025

The Optimization of a Protocol for the Directed Differentiation of Induced Pluripotent Stem Cells into Liver Progenitor Cells and the Delivery of Transgenes.

Applications

Unspecified application

Species

Unspecified reactive species

Irina Panchuk,Valeriia Kovalskaia,Natalia Balinova,Oxana Ryzhkova,Svetlana Smirnikhina

Cell reports methods 5:101047 PubMed40359937

2025

Harmonizing TUNEL with multiplexed iterative immunofluorescence enriches spatial contextualization of cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Marc S Sherman,Thomas McMahon-Skates,Lindsey S Gaston,Sonya W Katzen,Joseph A Majzoub,Wolfram Goessling

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70272 PubMed39777689

2025

The C3/C3aR pathway exacerbates acetaminophen-induced mouse liver injury via upregulating podoplanin on the macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Zhanli Xie,Jiang Jiang,Fei Yang,Jingjing Han,Zhenni Ma,Tao Wen,Xia Bai

iScience 27:111448 PubMed39720536

2024

Accumulation of alpha-synuclein pathology in the liver exhibits post-translational modifications associated with Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Hallbeck,Sara Ekmark-Lewén,Philipp J Kahle,Martin Ingelsson,Juan F Reyes

Journal of translational medicine 22:1036 PubMed39558364

2024

Isolation and identification of patient-derived liver cancer stem cells and development of personalized treatment strategies.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Guo,Shuai Zhang,Weiping Zeng,Yan Liang,Jinghe Xie,ShouPei Liu,Yaqi Qiu,Yingjie Fu,Yimeng Ou,Keqiang Ma,Bailin Wang,Weili Gu,Yuyou Duan

PloS one 19:e0308464 PubMed39110702

2024

Microglia through MFG-E8 signaling decrease the density of degenerating neurons and protect the brain from the development of cortical infarction after stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Yuhsiang Wang,Hank Szuhan Chen,Meng-Chih Wu,Ya Lan Yang,Hwai-Lee Wang,Che-Wei Liu,Ted Weita Lai

International journal of molecular sciences 25: PubMed38732047

2024

The Blood-Brain Barrier Is Unaffected in the Mouse Model of Leigh Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Robin Reynaud-Dulaurier,Romain Clément,Sara Yjjou,Cassandra Cresson,Yasmina Saoudi,Mathilde Faideau,Michael Decressac

Nature communications 15:2869 PubMed38693144

2024

A patient-based iPSC-derived hepatocyte model of alcohol-associated cirrhosis reveals bioenergetic insights into disease pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Bani Mukhopadhyay,Cheryl Marietta,Pei-Hong Shen,Abdul Oiseni,Faridoddin Mirshahi,Maria Mazzu,Colin Hodgkinson,Eli Winkler,Qiaoping Yuan,Daniel Miranda,George Kunos,Arun J Sanyal,David Goldman

PloS one 18:e0292681 PubMed37819924

2023

TAT peptide at treatment-level concentrations crossed brain endothelial cell monolayer independent of receptor-mediated endocytosis or peptide-inflicted barrier disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Chih Wu,Eric Yuhsiang Wang,Ted Weita Lai
View all publications

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