AB151754

Anti-ALAD 抗体 [EPR10268(B)]

Anti-ALAD antibody [EPR10268(B)]

RabMAb
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Recombinant Monoclonal ALAD antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

別名を表示する

Delta-aminolevulinic acid dehydratase, ALADH, Porphobilinogen synthase, ALAD

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALAD antibody [EPR10268(B)] (AB151754)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALAD antibody [EPR10268(B)] (AB151754)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling ALAD with ab151754 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ALAD antibody [EPR10268(B)] (AB151754)

All lanes:

Western blot - Anti-ALAD antibody [EPR10268(B)] (ab151754) at 1/1000 dilution

Lane 1:

Red blood cell lysate at 10 µg

Lane 2:

Fetal liver lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 36 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR10268(B)

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

style

left-column

製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The ALAD also known as Aminolevulinate Dehydratase is an essential enzyme involved in the biosynthesis of heme facilitating the condensation of two molecules of aminolevulinic acid to form porphobilinogen. The enzyme has a molecular mass of approximately 36 kDa. It is ubiquitously expressed across various tissues with notable activity in the liver and erythroid precursor cells within the bone marrow where heme biosynthesis is essential for the production of hemoglobin.

Biological function summary

The enzyme plays an important role in the early steps of the heme biosynthetic pathway. ALAD forms part of a multi-step process critical for heme production which is an important component of hemoglobin myoglobin and various cytochromes. The enzyme operates as a metalloenzyme requiring zinc for its activity and is subject to regulation by metal ion availability and substrate concentrations. Alterations in its activity can have significant impacts on cellular iron utilization and red blood cell function.

Pathways

ALAD plays a critical role within the heme biosynthesis pathway linking amino acid metabolism to porphyrin and heme production. It connects tightly with other enzymes involved in this pathway such as porphobilinogen deaminase. ALAD's function ensures the steady supply of intermediates necessary for synthesizing heme which serves further purposes in electron transport and oxygen transport systems within cells.

The improper function of ALAD associates with conditions such as lead poisoning and acute intermittent porphyria. In lead poisoning lead ions inhibit ALAD activity resulting in the accumulation of its substrate aminolevulinic acid and subsequent disturbances in heme production. In acute intermittent porphyria mutations in ALAD or interacting proteins like porphobilinogen deaminase can influence enzyme efficiency and increase the risk of metabolic crises. Understanding ALAD's role offers potential therapeutic insights for managing these disorders.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.

See full target information ALAD

style

left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

NPJ Regenerative medicine 7:55 PubMed36151109

2022

ZO-2/Tjp2 suppresses Yap and Wwtr1/Taz-mediated hepatocyte to cholangiocyte transdifferentiation in the mouse liver.

Applications

Unspecified application

Species

Unspecified reactive species

Jianliang Xu,P Jaya Kausalya,Alicia Ghia Min Ong,Christine Meng Fan Goh,Safiah Mohamed Ali,Walter Hunziker

Frontiers in cell and developmental biology 10:806408 PubMed35813194

2022

Significance of Liver Zonation in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shizhe Yu,Jie Gao,Haoren Wang,Long Liu,Xudong Liu,Yuantong Xu,Jihua Shi,Wenzhi Guo,Shuijun Zhang

Haematologica 104:1756-1767 PubMed30765471

2019

Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Nunziata Maio,Ki Soon Kim,Gregory Holmes-Hampton,Anamika Singh,Tracey A Rouault

Cell metabolism 28:573-587.e13 PubMed30017355

2018

Serine Synthesis via PHGDH Is Essential for Heme Production in Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Saar Vandekeere,Charlotte Dubois,Joanna Kalucka,Mark R Sullivan,Melissa García-Caballero,Jermaine Goveia,Rongyuan Chen,Frances F Diehl,Libat Bar-Lev,Joris Souffreau,Andreas Pircher,Saran Kumar,Stefan Vinckier,Yoshio Hirabayashi,Shigeki Furuya,Luc Schoonjans,Guy Eelen,Bart Ghesquière,Eli Keshet,Xuri Li,Matthew G Vander Heiden,Mieke Dewerchin,Peter Carmeliet

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALAD 抗体 [EPR10268(B)]

Anti-ALAD antibody [EPR10268(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALAD antibody [EPR10268(B)] (AB151754)

Western blot - Anti-ALAD antibody [EPR10268(B)] (AB151754)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

Abcam product promise