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Anti-AKT3 抗体 [66C1247.1] (ab118509)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 antibody [66C1247.1] (ab118509)

    Key features and details

    • Mouse monoclonal [66C1247.1] to AKT3
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-AKT3 antibody [66C1247.1]
      AKT3 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [66C1247.1] to AKT3

    • 由来種

      Mouse

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat, Chicken, Cow, Dog

    • 免疫原

      Synthetic peptide corresponding to Human AKT3 aa 100-200.
      Database link: Q9Y243

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • Human Brain Cerebellum tisse

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab118509の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    特記事項
    IHC-P
    Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    ターゲット情報

    • 機能

      IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.

    • 組織特異性

      In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.

    • 配列類似性

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.

    • ドメイン

      Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.

    • 翻訳後修飾

      Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
      Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.

    • 細胞内局在

      Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • AKT antibody
      • Akt3 antibody
      • AKT3 kinase antibody
      • AKT3_HUMAN antibody
      • DKFZp434N0250 antibody
      • MPPH antibody
      • PKB gamma antibody
      • PKBG antibody
      • PRKBG antibody
      • Protein kinase Akt-3 antibody
      • Protein Kinase AKT3 antibody
      • Protein kinase B gamma antibody
      • RAC gamma antibody
      • RAC gamma serine/threonine protein kinase antibody
      • RAC-gamma serine/threonine-protein kinase antibody
      • RAC-PK-gamma antibody
      • RACPK Gamma antibody
      • Serine threonine protein kinase Akt 3 antibody
      • Serine threonine protein kinase Akt3 antibody
      • STK 2 antibody
      • STK-2 antibody
      • STK2 antibody
      • V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody
      • V akt murine thymoma viral oncogene homolog 3 antibody
      • V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 antibody [66C1247.1] (ab118509)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 antibody [66C1247.1] (ab118509)
      ab118509 at 5 µg/ml staining AKT3 in Formalin-Fixed, Paraffin-Embedded Human Brain, Cerebellum tissue, followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

    参考文献 (0)

    ab118509 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab118509 は論文での使用が確認できていません。

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