Anti-AKT1 + AKT2 + AKT3 抗体 [EPR17671] (ab185633)
![Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)](https://www.abcam.co.jp/ps/products/185/ab185633/Images/ab185633-237228-anti-akt1-2-3-antibody-epr17671-western-blot.jpg "Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17671] to AKT1 + AKT2 + AKT3
- Suitable for: Flow Cyt (Intra), IP, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671]
AKT1 + AKT2 + AKT3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR17671] to AKT1 + AKT2 + AKT3 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IP, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: AKT3 recombinant protein fragment (His-Tag®): aa351-479; AKT2 recombinant protein fragment (His-Tag®): aa282-481; AKT1 recombinant protein fragment (His-Tag®): aa281-480; A549 whole cell lysate; Human fetal brain and fetal kidney lysates; Mouse brain lysate; Rat brain and heart lysates. IHC-P: Human cerebral cortex, Human adenocarcinoma of colon, mouse cerebral cortex and rat kidney tissues. ICC/IF: HeLa cells. Flow Cyt (intra): A549 cells. IP: A549 whole cell extract.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR17671 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185633の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IP |
1/50.
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| WB |
1/2000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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| IHC-P |
1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IP
1/50. |
|
WB
1/2000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
|
IHC-P
1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent. -
組織特異性
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
ドメイン
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. -
翻訳後修飾
Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. -
細胞内局在
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. - Information by UniProt
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参照データベース
- Entrez Gene: 10000 Human
- Entrez Gene: 207 Human
- Entrez Gene: 208 Human
- Entrez Gene: 11651 Mouse
- Entrez Gene: 11652 Mouse
- Entrez Gene: 23797 Mouse
- Entrez Gene: 24185 Rat
- Entrez Gene: 25233 Rat
see all -
別名
- AKT antibody
- AKT1 antibody
- AKT1 kinase antibody
see all
画像
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Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633) at 1/5000 dilution
Lane 1 : AKT3 recombinant protein fragment (His-Tag®): aa351-479
Lane 2 : AKT2 recombinant protein fragment (His-Tag®): aa282-481
Lane 3 : AKT1 recombinant protein fragment (His-Tag®): aa281-480
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
The bottom panel is probed with an anti-His tag antibody.
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![Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)](https://www.abcam.co.jp/ps/products/185/ab185633/Images/ab185633-237229-anti-akt1-2-3-antibody-epr17671-western-blot.jpg)
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633) at 1/2000 dilution + A549 (Human lung carcinoma) whole cell lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)](https://www.abcam.co.jp/ps/products/185/ab185633/Images/ab185633-237230-anti-akt1-2-3-antibody-epr17671-western-blot.jpg)
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633) at 1/2000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : Human fetal kidney lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)](https://www.abcam.co.jp/ps/products/185/ab185633/Images/ab185633-237231-anti-akt1-2-3-antibody-epr17671-western-blot.jpg)
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633) at 1/2000 dilution
Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lane 3 : Rat heart lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)](https://www.abcam.co.jp/ps/products/185/ab185633/Images/ab185633-237223-anti-akt1-2-3-antibody-epr17671-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling AKT1 + AKT2 + AKT3 with ab185633 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and weak cytoplasmic staining on neurons of the Human cerebral cortex is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)](https://www.abcam.co.jp/ps/products/185/ab185633/Images/ab185633-237224-anti-akt1-2-3-antibody-epr17671-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of colon tissue labeling AKT1 + AKT2 + AKT3 with ab185633 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and weak cytoplasmic staining on Human adenocarcinoma of colon is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)](https://www.abcam.co.jp/ps/products/185/ab185633/Images/ab185633-237225-anti-akt1-2-3-antibody-epr17671-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling AKT1 + AKT2 + AKT3 with ab185633 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and weak cytoplasmic staining on neurons of the mouse cerebral cortex is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)](https://www.abcam.co.jp/ps/products/185/ab185633/Images/ab185633-237226-anti-akt1-2-3-antibody-epr17671-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling AKT1 + AKT2 + AKT3 with ab185633 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and weak cytoplasmic staining on rat kidney is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunoprecipitation - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)](https://www.abcam.co.jp/ps/products/185/ab185633/Images/ab185633-237227-anti-akt1-2-3-antibody-epr17671-immunoprecipitation.jpg)
Immunoprecipitation - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)AKT1 + AKT2 + AKT3 was immunoprecipitated from 1mg of A549 (Human lung carcinoma) whole cell extract with ab185633 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab185633 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: A549 whole cell extract 10 µg (Input). Lane 2: ab185633 IP in A549 whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab185633 in A549 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST. -
![Flow Cytometry (Intracellular) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)](https://www.abcam.co.jp/ps/products/185/ab185633/Images/ab185633-237221-anti-akt1-2-3-antibody-epr17671-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedA549 (Human lung carcinoma) cells labeling AKT1 + AKT2 + AKT3 with ab185633 at 1/50 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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![Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)](https://www.abcam.co.jp/ps/products/185/ab185633/Images/ab185633-12-benefits-of-recombinant-antibodies.png)
Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (12)
ab185633 は 12 報の論文で使用されています。
- Wang Y et al. Pretreatment with rosavin attenuates PM2.5-induced lung injury in rats through antiferroptosis via PI3K/Akt/Nrf2 signaling pathway. Phytother Res 37:195-210 (2023). PubMed: 36097321
- Han X & Zhuang Y PM2.5 induces autophagy-mediated cell apoptosis via PI3K/AKT/mTOR signaling pathway in mice bronchial epithelium cells. Exp Ther Med 21:1 (2021). PubMed: 33235610
- Pei C et al. Astragaloside IV Protects from PM2.5-Induced Lung Injury by Regulating Autophagy via Inhibition of PI3K/Akt/mTOR Signaling in vivo and in vitro. J Inflamm Res 14:4707-4721 (2021). PubMed: 34557015
- Wu Y et al. Exploring the Pharmacological Mechanisms of Xihuang Pills Against Prostate Cancer via Integrating Network Pharmacology and Experimental Validation In Vitro and In Vivo. Front Pharmacol 12:791269 (2021). PubMed: 35342388
- Kwakye AK et al. Levobupivacaine inhibits proliferation and promotes apoptosis of breast cancer cells by suppressing the PI3K/Akt/mTOR signalling pathway. BMC Res Notes 13:386 (2020). PubMed: 32807213