Anti-AKT (phospho T308) 抗体 (ab38449)
Key features and details
- Rabbit polyclonal to AKT (phospho T308)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-AKT (phospho T308) antibody -
製品の詳細
Rabbit polyclonal to AKT (phospho T308) -
由来種
Rabbit -
特異性
This antibody was made against a peptide directed against the phosphorylated form of AKT1 at T308, but due to a high degree of homology it is predicted to cross react with AKT2 and AKT3 if they are phosphorylated at the corresponding residue.
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アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human AKT aa 250-350 (phospho T308).
Database link: P31749 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab38449の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
1/500 - 1/1000. Predicted molecular weight: 56 kDa.
Block in 5% non-fat milk in TBST, RT, 90min. In order to detect a clear signal, treatment is required when using this antibody. |
IHC-P | (1) |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 56 kDa. Block in 5% non-fat milk in TBST, RT, 90min. In order to detect a clear signal, treatment is required when using this antibody. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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関連性
AKT, also known as protein kinase B (PKB), is a serine/threonine protein kinase. There are three mammalian isoforms of AKT: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. AKT has been shown to play a role in such metabolic processes as glucose transport, glycogen synthesis, glycolysis, and protein synthesis. It had also been shown to promote cell survival by inhibiting apoptosis through its ability to phosphoylate and inactivate several targets, including Bad, Forkhead transcription factors, and caspase 9. Activity of AKT has been associated with the phosphorylation of two sites: T308, in the activation loop of the kinase, and S473, at the carboxyl terminus. Phosphorylation of both sites contributes to AKT activity, however phosphorylation of T308 has been shown to be absolutely essential for AKT activation. -
細胞内局在
Cell Membrane, Cytoplasmic and Nuclear. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1). -
参照データベース
- Entrez Gene: 207 Human
- Entrez Gene: 11651 Mouse
- Omim: 164730 Human
- SwissProt: P31749 Human
- SwissProt: P31750 Mouse
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別名
- AKT1 antibody
- C AKT antibody
- PKB antibody
see all
画像
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All lanes : Anti-AKT (phospho T308) antibody (ab38449) at 1/1000 dilution
Lane 1 : HeLa cells
Lane 2 : HeLa cells treated with 0.01 U/mL Insulin for 15 minutes
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution
Predicted band size: 56 kDa10% gel.
Running conditions: 60v, 30min; 120v 60min
Transfer conditions: 150mA 120min Nitrocellulose membrane.
Blocking conditions: 5% non-fat milk in TBST, RT, 90min.
Primary antibody incubation: 4?, overnight
Secondary antibody incubation: room temperature for 45 minutes
Washing condition: 5 ml TBST, 4 x 5min
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Immunohistochemical analysis of AKT (phospho T308) expression in paraffin embedded human lung carcinoma tissue, using ab38449 (1/50). Right-hand panel represents a negative control where ab38449 was pre-incubated with the immunizing (blocking) peptide.
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All lanes : Anti-AKT (phospho T308) antibody (ab38449) at 1/500 dilution
Lane 1 : 293 cell lysate - untreated
Lane 2 : 293 cell lysate - treated with insulin
Lysates/proteins at 30 µg per lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (778)
ab38449 は 778 報の論文で使用されています。
- Liu D et al. Pachymic acid (PA) inhibits ferroptosis of cardiomyocytes via activation of the AMPK in mice with ischemia/reperfusion-induced myocardial injury. Cell Biol Int 48:46-59 (2024). PubMed: 37750505
- Yang S et al. Neutrophil extracellular traps promote angiogenesis in gastric cancer. Cell Commun Signal 21:176 (2023). PubMed: 37480055
- Park KH et al. Activation of MG53 Enhances Cell Survival and Engraftment of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Injured Hearts. Stem Cell Rev Rep 19:2420-2428 (2023). PubMed: 37477774
- Zhu M et al. LRP1 as a promising therapeutic target for gastrointestinal tumors: Inhibiting proliferation, invasion and migration of cancer cells. Oncol Lett 26:432 (2023). PubMed: 37664649
- Zhang Y et al. Saikosaponin A alleviates glycolysis of breast cancer cells through repression of Akt/STAT3 pathway. Chem Biol Drug Des 102:115-125 (2023). PubMed: 37088850