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リコンビナントRabMAb

Anti-AKAP14 抗体 [EPR12955] - C-terminal (ab180160)

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Western blot - Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)
  • Immunocytochemistry/ Immunofluorescence - Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)
  • Immunoprecipitation - Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)
  • Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12955] to AKAP14 - C-terminal
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-AKAP14 antibody [EPR12955] - C-terminal
    AKAP14 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12955] to AKAP14 - C-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IP, ICC/IFmore details
    適用なし: Flow Cyt or IHC-P

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Jurkat, HUVEC, NCI-H460, A549 and Human fetal kidney lysates; A549 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR12955

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab180160の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IP
1/10 - 1/100.
ICC/IF
1/100 - 1/250.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IP
1/10 - 1/100.
ICC/IF
1/100 - 1/250.
追加情報
Is unsuitable for Flow Cyt or IHC-P.

ターゲット情報

  • 関連性

    Binds to type II regulatory subunits of protein kinase A and anchors/targets them

  • 細胞内局在

    Cytoplasmic

  • 参照データベース

    • 別名

      • A kinase (PRKA) anchor protein 14 antibody
      • A kinase anchor protein 14 antibody
      • A kinase anchor protein 28 kDa antibody
      • A-kinase anchoring protein 28 antibody
      • AKAP14 antibody
      • AKAP28 antibody
      • PRKA14 antibody
      • Protein kinase A anchoring protein 14 antibody
      see all

    画像

    • Western blot - Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)
      Western blot - Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)
      All lanes : Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HUVEC cell lysate
      Lane 3 : NCI-H460 cell lysate
      Lane 4 : A549 cell lysate
      Lane 5 : Human fetal kidney tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 23 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)
      Immunocytochemistry/ Immunofluorescence - Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)

      Immunofluorescence analysis of A549 cells labeling AKAP14 with ab180160 at a 1/100 dilution.

    • Immunoprecipitation - Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)
      Immunoprecipitation - Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)

      Western blot analysis on Immunoprecipitation pellet from either 1) Jurkat cell lysate, or 2) 1xPBS (negative control); showing AKAP14, using ab180160 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)
      Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)

    参考文献 (0)

    ab180160 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab180160 は論文での使用が確認できていません。

    レビューと Q&A

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