Anti-AK5 抗体 [OTI1D6] (ab117927)
![Western blot - Anti-AK5 antibody [OTI1D6] (ab117927)](https://www.abcam.co.jp/ps/products/117/ab117927/Images/AK5-Primary-antibodies-ab117927-1.jpg "Western blot - Anti-AK5 antibody [OTI1D6] (ab117927)")
Key features and details
- Mouse monoclonal [OTI1D6] to AK5
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human, African green monkey
- Isotype: IgG1
製品の概要
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製品名
Anti-AK5 antibody [OTI1D6]
AK5 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [OTI1D6] to AK5 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, Flow Cytmore details -
種交差性
交差種: Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human AK5 aa 1-600. NP_777283. Produced in HEK-293T cells.
Database link: Q9Y6K8 -
ポジティブ･コントロール
- WB: HEK-293T cells transfected with pCMV6-ENTRY AK5 cDNA. ICC/IF: COS-7 transiently transfected by pCMV6-ENTRY AK5 cDNA. Flow Cyt: HEK-293T cells transfected with AK5 (Myc-DDK-tagged)-Human adenylate kinase 5 (AK5), transcript variant 1.
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特記事項
Clone OTI1D6 (formerly 1D6).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Purified from cell culture supernatant by affinity chromatography. -
ポリ/モノ
モノクローナル -
クローン名
OTI1D6 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117927の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 63 kDa.
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| ICC/IF |
1/100.
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| Flow Cyt |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
1/2000. Predicted molecular weight: 63 kDa. |
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ICC/IF
1/100. |
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Flow Cyt
Use at an assay dependent concentration. |
ターゲット情報
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機能
Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. -
組織特異性
Brain specific. -
配列類似性
Belongs to the adenylate kinase family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 26289 Human
- Omim: 608009 Human
- SwissProt: Q9Y6K8 Human
- Unigene: 559718 Human
- Unigene: 597002 Human
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別名
- Adenylate kinase 5 antibody
- Adenylate kinase 6 antibody
- Adenylate kinase isoenzyme 5 antibody
see all
画像
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Western blot - Anti-AK5 antibody [OTI1D6] (ab117927)All lanes : Anti-AK5 antibody [OTI1D6] (ab117927) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY AK5 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 63 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-AK5 antibody [OTI1D6] (ab117927)](https://www.abcam.co.jp/ps/products/117/ab117927/Images/AK5-Primary-antibodies-ab117927-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AK5 antibody [OTI1D6] (ab117927)pCMV6-ENTRY AK5 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for AK5 using ab117927 at a 1/100 dilution in ICC/IF.
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![Flow Cytometry - Anti-AK5 antibody [OTI1D6] (ab117927)](https://www.abcam.co.jp/ps/products/117/ab117927/Images/ab117927-314116-117927777.jpg)
Flow Cytometry - Anti-AK5 antibody [OTI1D6] (ab117927)Flow Cytometry analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with AK5 (Myc-DDK-tagged)-Human adenylate kinase 5 (AK5), transcript variant 1, using ab117927 (red) compared to an empty vector control plasmid (blue).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab117927 は論文での使用が確認できていません。