Anti-AIMP3/p18 抗体 (ab31543)
Key features and details
- Rabbit polyclonal to AIMP3/p18
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-AIMP3/p18 antibody
AIMP3/p18 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AIMP3/p18 -
由来種
Rabbit -
特異性
ab31543 reacts specifically with 21kDa p18 and immunoprecipitates with ATM/ATR -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human AIMP3/p18.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31543の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/8000. Detects a band of approximately 21 kDa (predicted molecular weight: 18 kDa).
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| ICC/IF |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
1/8000. Detects a band of approximately 21 kDa (predicted molecular weight: 18 kDa). |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Positive modulator of ATM response to DNA damage. -
組織特異性
Down-regulated in various cancer tissues. -
配列類似性
Contains 1 GST C-terminal domain. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasmic under growth arrest conditions. Translocated into the nucleus when growth resumes (S phase) and following DNA damage. - Information by UniProt
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参照データベース
- Entrez Gene: 9521 Human
- Omim: 609206 Human
- SwissProt: O43324 Human
- Unigene: 602353 Human
- Unigene: 726163 Human
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別名
- AIMP 3 antibody
- AIMP3 antibody
- Aminoacyl tRNA synthetase complex interacting multifunctional protein 3 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-AIMP3/p18 antibody (ab31543)
Immunofluorescence analysis of Hela cells using anti-AIMP3 (p18) antibody (ab31543).
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Western blot - Anti-AIMP3/p18 antibody (ab31543)Anti-AIMP3/p18 antibody (ab31543) at 1/20000 dilution + HEK293 cell line
Predicted band size: 18 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-AIMP3/p18 antibody (ab31543)Immunofluorescence analysis of U2OS cells using anti-AIMP3 (p18) antibody (ab31543).
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Western blot - Anti-AIMP3/p18 antibody (ab31543)Anti-AIMP3/p18 antibody (ab31543) at 1/8000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml
Secondary
Goat anti-rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 18 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab31543 は 6 報の論文で使用されています。
- Jin D et al. HIV-1 Gag Binds the Multi-Aminoacyl-tRNA Synthetase Complex via the EPRS Subunit. Viruses 15:N/A (2023). PubMed: 36851687
- Wang K et al. Proteomics and metabolomics of raw rhubarb and wine-processed rhubarb in the treatment of rats with intracerebral hemorrhage. Ann Transl Med 8:1670 (2020). PubMed: 33490182
- Gulati P et al. Role for the obesity-related FTO gene in the cellular sensing of amino acids. Proc Natl Acad Sci U S A 110:2557-62 (2013). WB ; Mouse . PubMed: 23359686
- Du Y et al. Elevation of Highly Up-regulated in Liver Cancer (HULC) by Hepatitis B Virus X Protein Promotes Hepatoma Cell Proliferation via Down-regulating p18. J Biol Chem 287:26302-11 (2012). WB ; Human . PubMed: 22685290
- David A et al. RNA Binding Targets Aminoacyl-tRNA Synthetases to Translating Ribosomes. J Biol Chem 286:20688-700 (2011). Human . PubMed: 21460219
- Oh YS et al. Downregulation of lamin A by tumor suppressor AIMP3/p18 leads to a progeroid phenotype in mice. Aging Cell 9:810-22 (2010). WB ; Mouse . PubMed: 20726853