Anti-Ahsp 抗体 [EPR12319] (ab180861)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody [EPR12319] (ab180861)](https://www.abcam.co.jp/ps/products/180/ab180861/Images/ab180861-209741-anti-ahsp-antibody-epr12319-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody [EPR12319] (ab180861)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12319] to Ahsp
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Ahsp antibody [EPR12319]
Ahsp 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12319] to Ahsp -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IPmore details
適用なし: ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human fetal liver lysate; Human fetal spleen tissue, Human lung tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12319 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180861の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 12 kDa.
|
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| IHC-P | (1) |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| IP |
1/10 - 1/100.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 12 kDa. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
IP
1/10 - 1/100. |
ターゲット情報
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機能
Act as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia. -
組織特異性
Expressed in blood and bone marrow. -
配列類似性
Belongs to the AHSP family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 51327 Human
- Omim: 605821 Human
- SwissProt: Q9NZD4 Human
- Unigene: 274309 Human
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別名
- AHSP antibody
- AHSP_HUMAN antibody
- Alpha hemoglobin stabilizing protein antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody [EPR12319] (ab180861)
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Ahsp with ab180861 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Ahsp antibody [EPR12319] (ab180861)](https://www.abcam.co.jp/ps/products/180/ab180861/Images/ab180861-209742-anti-ahsp-antibody-epr12319-western-blot.jpg)
Western blot - Anti-Ahsp antibody [EPR12319] (ab180861)Anti-Ahsp antibody [EPR12319] (ab180861) at 1/1000 dilution + Human fetal liver tissue lysate at 10 µg
Predicted band size: 12 kDa -
![Immunoprecipitation - Anti-Ahsp antibody [EPR12319] (ab180861)](https://www.abcam.co.jp/ps/products/180/ab180861/Images/ab180861-209744-anti-ahsp-antibody-epr12319-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Ahsp antibody [EPR12319] (ab180861)Western blot analysis of immunoprecipitation pellet from Human fetal liver lysate immunoprecipitated using ab180861 at 1/10 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody [EPR12319] (ab180861)](https://www.abcam.co.jp/ps/products/180/ab180861/Images/ab180861-209740-anti-ahsp-antibody-epr12319-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody [EPR12319] (ab180861)Immunohistochemical analysis of paraffin-embedded Human fetal spleen tissue labeling Ahsp with ab180861 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Ahsp antibody [EPR12319] (ab180861)](https://www.abcam.co.jp/ps/products/180/ab180861/Images/ab180861-5-benefits-of-recombinant-antibodies.png)
Anti-Ahsp antibody [EPR12319] (ab180861)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab180861 は論文での使用が確認できていません。