AB187051

Anti-AHSG 抗体 [EPR17839-163]

Anti-AHSG antibody [EPR17839-163]

20ul selling size
RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal FETUA antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat samples. Cited in 6 publications.

別名を表示する

Fetua, Ahsg, Alpha-2-HS-glycoprotein, Countertrypin, Fetuin-A

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHSG antibody [EPR17839-163] (AB187051)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHSG antibody [EPR17839-163] (AB187051)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling AHSG with ab187051 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat liver (PMID : 24604240; PMID : 12943536; PMID : 22170794) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHSG antibody [EPR17839-163] (AB187051)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling AHSG with ab187051 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse liver (PMID : 24604240; PMID : 12943536; PMID : 22170794) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Supplier Data

Immunoprecipitation - Anti-AHSG antibody [EPR17839-163] (AB187051)

AHSG was immunoprecipitated from 0.35 mg of mouse serum with ab187051 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187051 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : Mouse serum 10 μg (Input).
Lane 2 : ab187051 IP in mouse serum (+).
Lane 3 : : Rabbit monoclonal IgG (ab172730) instead of ab187051 in mouse serum (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-AHSG antibody [EPR17839-163] (ab187051)

Predicted band size: 39 kDa

true

Supplier Data

Western blot - Anti-AHSG antibody [EPR17839-163] (AB187051)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-AHSG antibody [EPR17839-163] (ab187051) at 1/2000 dilution

Lane 1:

Mouse serum at 20 µg

Lane 2:

Mouse plasma at 20 µg

Lane 3:

Rat serum at 20 µg

Lane 4:

Rat plasma at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

Observed band size: 58 kDa

true

Exposure time: 5s

Supplier Data

Western blot - Anti-AHSG antibody [EPR17839-163] (AB187051)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-AHSG antibody [EPR17839-163] (ab187051) at 1/2000 dilution

All lanes:

Rat liver tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

Observed band size: 58 kDa

true

Exposure time: 1min

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR17839-163

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat

アプリケーション

IP, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The target AHSG also known as fetuin-A or alpha-2-HS-glycoprotein is a multifunctional glycoprotein with an approximate mass of 64-68 kDa. It is mainly expressed in the liver and secreted into the bloodstream. AHSG exhibits a wide range of interactions with different cell types and biological molecules playing key roles in mineralization inhibition of arterial calcification and modulation of immune responses.

Biological function summary

AHSG protein serves multiple functions including acting as an inhibitor of insulin receptor autophosphorylation and serine-threonine kinase activities. AHSG is part of a complex dynamic process involving calcium metabolism and bone mineralization. It binds to transforming growth factor-beta (TGF-β) and other proteins modulating cellular signals and maintaining homeostasis in tissues. The regulation of calcium ion balance by AHSG can protect against ectopic calcification preventing potential damage.

Pathways

Calcium signaling and the insulin signaling pathways integrate AHSG functions. AHSG interacts with proteins like insulin receptor and those in the canonical Wnt signaling pathway influencing glucose metabolism and bone development. The balance of its interactions in these pathways helps maintain metabolic homeostasis and skeletal integrity reflecting its versatile roles within complex networks.

AHSG has connections to metabolic syndrome and calcific aortic valve disease. Abnormal levels of AHSG can disrupt metabolic functions and contribute to type 2 diabetes development by affecting insulin receptor activity. It also associates with calcification-related proteins in vascular tissues linking it to cardiovascular diseases. Understanding these connections is important for potential therapeutic targeting of AHSG in related pathologies.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Probably involved in differentiation.. (Microbial infection) Facilitates invasion of hepatocytes by Plasmodium berghei sporozoites.

See full target information Ahsg

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of diabetes research 2025:2379872 PubMed41069927

2025

Transcriptomics and Proteomics Reveal That TLPW Acupuncture Ameliorates Proteinuria in Diabetic Kidney Disease Model Rats by Suppressing Epithelial-to-Mesenchymal Transition via the DPP4/SDF-1/TGF-/Smad Signalling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Ji,Jiya Sun,Zihao Zhuang,Yunming Xiao,Shipian Li,Zhilong Zhang,Xu Wang,Xinju Li

Journal of thoracic disease 16:6024-6036 PubMed39444871

2024

Ethyl palmitate ameliorates lethal endotoxemia by inducing hepatic fetuin-A secretion: an and experiment.

Applications

Unspecified application

Species

Unspecified reactive species

Guannan Chen,Jieqiong Song,Yue Zhou,Yuanyuan Wu,Chenghang Yang,Yingqin Wang,Ming Zhong

Journal of neuroinflammation 19:269 PubMed36333786

2022

Fetuin-A alleviates neuroinflammation against traumatic brain injury-induced microglial necroptosis by regulating Nrf-2/HO-1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Pengzhan Zhao,Yutian Wei,Guangchi Sun,Lei Xu,Tian Wang,Yufei Tian,Honglu Chao,Yiming Tu,Jing Ji

Frontiers in immunology 13:837991 PubMed35359973

2022

A Metabolism-Related Gene Landscape Predicts Prostate Cancer Recurrence and Treatment Response.

Applications

Unspecified application

Species

Unspecified reactive species

Lijie Zhou,Ruixin Fan,Yongbo Luo,Cai Zhang,Donghui Jia,Rongli Wang,Youmiao Zeng,Mengda Ren,Kaixuan Du,Wenbang Pan,Jinjian Yang,Fengyan Tian,Chaohui Gu

Oxidative medicine and cellular longevity 2021:8899274 PubMed34007409

2021

Proteomic Analysis Revealed the Characteristics of Key Proteins Involved in the Regulation of Inflammatory Response, Leukocyte Transendothelial Migration, Phagocytosis, and Immune Process during Early Lung Blast Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yunen Liu,Changci Tong,Peifang Cong,Ying Liu,Xiuyun Shi,Lin Shi,Shun Mao,Yan Zhao,Hongxu Jin,Mingxiao Hou

Biochemical pharmacology 166:46-55 PubMed31077645

2019

Hepatic upregulation of fetuin-A mediates acetaminophen-induced liver injury through activation of TLR4 in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kang-Yo Lee,Wonseok Lee,Seung-Hwan Jung,Jungmin Park,Hyungtai Sim,You-Jin Choi,Young-Jun Park,Yeonseok Chung,Byung-Hoon Lee

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AHSG 抗体 [EPR17839-163]

Anti-AHSG antibody [EPR17839-163]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHSG antibody [EPR17839-163] (AB187051)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHSG antibody [EPR17839-163] (AB187051)

Immunoprecipitation - Anti-AHSG antibody [EPR17839-163] (AB187051)

Western blot - Anti-AHSG antibody [EPR17839-163] (AB187051)

Western blot - Anti-AHSG antibody [EPR17839-163] (AB187051)

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (6)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise