Anti-AHA1 抗体 (ab83036)
Key features and details
- Rabbit polyclonal to AHA1
- Suitable for: ELISA, ICC, IHC-Fr, IHC-P, IP, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-AHA1 antibody
AHA1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AHA1 -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, ICC, IHC-Fr, IHC-P, IP, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Full length protein corresponding to Mouse AHA1 aa 1-350.
Database link: Q8BK64 -
ポジティブ・コントロール
- WB: A431 cells, A549 cells, HCT116 cells, Hela cells, HEK293 cells, HepG2 cells, HL-60 cells, HUVEC cells, Jurkat cells, MCF7 cells, PC3 cells, T98G cells, rat brain ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab83036の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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ICC |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/2000. Predicted molecular weight: 38 kDa.
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ICC/IF |
1/60.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
1/1000 - 1/2000. Predicted molecular weight: 38 kDa. |
ICC/IF
1/60. |
ターゲット情報
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機能
Cochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking. -
組織特異性
Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta. -
配列類似性
Belongs to the AHA1 family. -
細胞内局在
Cytoplasm > cytosol. Endoplasmic reticulum. May transiently interact with the endoplasmic reticulum. - Information by UniProt
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参照データベース
- Entrez Gene: 10598 Human
- Entrez Gene: 217737 Mouse
- Omim: 608466 Human
- SwissProt: O95433 Human
- SwissProt: Q8BK64 Mouse
- Unigene: 204041 Human
- Unigene: 22626 Mouse
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別名
- Activator of 90 kDa heat shock protein ATPase homolog 1 antibody
- Activator of heat shock 90kDa protein ATPase homolog 1 antibody
- AHA 1 antibody
see all
画像
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Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AHA1 Polyclonal Antibody (ab83036). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-AHA1 Polyclonal Antibody (ab83036) at 1:60 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Endoplasmic reticulum. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-AHA1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
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All lanes : Anti-AHA1 antibody (ab83036)
Lane 1 : Cell lysates prepared from A431 cells
Lane 2 : Cell lysates prepared from A549 cells
Lane 3 : Cell lysates prepared from HCT116 cells
Lane 4 : Cell lysate prepared from Hela cells
Lane 5 : Cell lysate prepared from HEK293 cells
Lane 6 : Cell lysates prepared from HepG2 cells
Lane 7 : Cell lysates prepared from HL-60 cells
Lane 8 : Cell lysates prepared from HUVEC cells
Lane 9 : Cell lysates prepared from Jurkat cells
Lane 10 : Cell lysates prepared from MCF7 cells
Lane 11 : Cell lysates prepared from PC3 cells
Lane 12 : Cell lysates prepared from T98G cells
Lane 13 : Lysate prepared from rat brain
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab83036 は 4 報の論文で使用されています。
- Heider M et al. The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma. Mol Cell 81:1170-1186.e10 (2021). PubMed: 33571422
- Shelton LB et al. Hsp90 activator Aha1 drives production of pathological tau aggregates. Proc Natl Acad Sci U S A 114:9707-9712 (2017). PubMed: 28827321
- Ghosh S et al. Endoplasmic Reticulum Resident Heat Shock Protein 90 (Hsp90) Isoform Glucose-Regulated Protein 94 (Grp94) Regulates Cell Polarity and Cancer Cell Migration by Affecting Intracellular Transport. J Biol Chem N/A:N/A (2016). WB . PubMed: 26872972
- Moura CS et al. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS One 9:e83437 (2014). WB ; Rat . PubMed: 24465380