AB83036

Anti-AHA1 抗体

Anti-AHA1 antibody

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(4 Publications)

Rabbit Polyclonal AHA1 antibody. Suitable for ICC, IP, ELISA, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Full Length Protein corresponding to Mouse Ahsa1 aa 1 to C-terminus.

別名を表示する

C14orf3, HSPC322, AHSA1, Activator of 90 kDa heat shock protein ATPase homolog 1, AHA1, p38

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-AHA1 antibody (AB83036)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AHA1 antibody (AB83036)

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AHA1 Polyclonal Antibody (ab83036). Tissue : Heat Shocked Cervical cancer cell line (HeLa). Species : Human. Fixation : 2% Formaldehyde for 20 min at RT. Primary Antibody : Rabbit Anti-AHA1 Polyclonal Antibody (ab83036) at 1 : 60 for 12 hours at 4°C. Secondary Antibody : R-PE Goat Anti-Rabbit (yellow) at 1 : 200 for 2 hours at RT. Counterstain : DAPI (blue) nuclear stain at 1 : 40000 for 2 hours at RT. Localization : Cytoplasm. Endoplasmic reticulum. Magnification : 100x. (A) DAPI (blue) nuclear stain. (B) Anti-AHA1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Unknown

Western blot - Anti-AHA1 antibody (AB83036)

All lanes:

Western blot - Anti-AHA1 antibody (ab83036)

Lane 1:

Cell lysates prepared from A431 cells at 10 µg

Lane 2:

Cell lysates prepared from A549 cells at 10 µg

Lane 3:

Cell lysates prepared from HCT116 cells at 10 µg

Lane 4:

Cell lysate prepared from Hela cells at 10 µg

Lane 5:

Cell lysate prepared from HEK293 cells at 10 µg

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Cell lysates prepared from HepG2 cells at 10 µg

Lane 7:

Cell lysates prepared from HL-60 cells at 10 µg

Lane 8:

Cell lysates prepared from HUVEC cells at 10 µg

Lane 9:

Cell lysates prepared from Jurkat cells at 10 µg

Lane 10:

Cell lysates prepared from MCF7 cells at 10 µg

Lane 11:

Cell lysates prepared from PC3 cells at 10 µg

Lane 12:

Cell lysates prepared from T98G cells at 10 µg

Lane 13:

Lysate prepared from rat brain at 10 µg

Predicted band size: 38 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Human, Mouse

アプリケーション

IHC-Fr, WB, ICC, IHC-P, IP, ICC/IF, ELISA

applications

免疫原

Full Length Protein corresponding to Mouse Ahsa1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8BK64

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000.00000 - 1/2000.00000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/60", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49.1% PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

AHA1 also known as activator of heat shock 90kDa protein ATPase homolog 1 is a co-chaperone protein that plays a role in the regulation of HSP90 ATPase activity. This protein has a molecular weight of approximately 38 kDa. AHA1 expression occurs in various tissues with higher amounts seen in metabolically active tissues. It interacts directly with HSP90 enhancing the chaperone's ability to fold client proteins correctly by stimulating its ATPase activity.

Biological function summary

AHA1 modulates the function of the HSP90 chaperone complex. This complex is important for the stabilization and activation of many client proteins such as steroid hormone receptors and kinases. AHA1 binds to the middle domain of HSP90 contributing to conformational changes necessary for efficient ATP hydrolysis. By influencing these molecular processes AHA1 plays a role in maintaining cellular protein homeostasis under stress conditions.

Pathways

The HSP90-AHA1 interaction is significant in the signaling pathway of cellular stress responses and protein folding. AHA1's upregulating effect on HSP90 ATPase activity facilitates the correct folding and function of client proteins involved in the MAPK/ERK and PI3K/AKT pathways. These pathways commonly involve interactions with proteins such as RAF and PI3K which are important in cell proliferation and survival processes.

AHA1 influences the progression of cancer and neurodegenerative diseases. Altered expression or function of AHA1 is linked to the development and progression of cancer as it affects the stability of oncoproteins through its interaction with HSP90. The protein is also connected to neurodegenerative diseases with disruptions in its function or expression impacting the folding and maintenance of neuronal proteins. In these diseases the AHA1-HSP90 interaction remains a critical component of pathogenic pathways affecting disease manifestation and progression.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Acts as a co-chaperone of HSP90AA1 (PubMed : 29127155). Activates the ATPase activity of HSP90AA1 leading to increase in its chaperone activity (PubMed : 29127155). Competes with the inhibitory co-chaperone FNIP1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed : 27353360). Competes with the inhibitory co-chaperone TSC1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed : 29127155).

See full target information AHSA1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 81:1170-1186.e10 PubMed33571422

2021

The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Heider,Ruth Eichner,Jacob Stroh,Volker Morath,Anna Kuisl,Jana Zecha,Jannis Lawatscheck,Kheewoong Baek,Anne-Kathrin Garz,Martina Rudelius,Friedrich-Christian Deuschle,Ulrich Keller,Simone Lemeer,Mareike Verbeek,Katharina S Götze,Arne Skerra,Wolfgang A Weber,Johannes Buchner,Brenda A Schulman,Bernhard Kuster,Vanesa Fernández-Sáiz,Florian Bassermann

Proceedings of the National Academy of Sciences of 114:9707-9712 PubMed28827321

2017

Hsp90 activator Aha1 drives production of pathological tau aggregates.

Applications

Unspecified application

Species

Unspecified reactive species

Lindsey B Shelton,Jeremy D Baker,Dali Zheng,Leia E Sullivan,Parth K Solanki,Jack M Webster,Zheying Sun,Jonathan J Sabbagh,Bryce A Nordhues,John Koren,Suman Ghosh,Brian S J Blagg,Laura J Blair,Chad A Dickey

The Journal of biological chemistry 291:8309-23 PubMed26872972

2016

Endoplasmic Reticulum-resident Heat Shock Protein 90 (HSP90) Isoform Glucose-regulated Protein 94 (GRP94) Regulates Cell Polarity and Cancer Cell Migration by Affecting Intracellular Transport.

Applications

Species

Unspecified reactive species

Suman Ghosh,Heather E Shinogle,Nadezhda A Galeva,Rick T Dobrowsky,Brian S J Blagg

PloS one 9:e83437 PubMed24465380

2014

Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats.

Applications

Species

Rat

Carolina Soares Moura,Pablo Christiano Barboza Lollo,Priscila Neder Morato,Luciana Hisayama Nisishima,Everardo Magalhães Carneiro,Jaime Amaya-Farfan

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

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Anti-AHA1 抗体

Anti-AHA1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-AHA1 antibody (AB83036)

Western blot - Anti-AHA1 antibody (AB83036)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

Abcam product promise

関連製品

Alternative Product

Primary Antibodies

Anti-AHA1 antibody