Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-AGXT 抗体 [EPR13232] (ab178708)

Submit a review Submit a question References (1)
Western blot - Anti-AGXT antibody [EPR13232] (ab178708)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] (ab178708)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] (ab178708)
  • Immunoprecipitation - Anti-AGXT antibody [EPR13232] (ab178708)
  • Western blot - Anti-AGXT antibody [EPR13232] (ab178708)
  • Anti-AGXT antibody [EPR13232] (ab178708)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13232] to AGXT
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-AGXT antibody [EPR13232]
    AGXT 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13232] to AGXT

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Recombinant fragment corresponding to Human AGXT.
    Database link: P21549

  • ポジティブ･コントロール

    • HepG2 and Human fetal liver lysates; Human hepatocellular carcinoma and Human liver tissue.

  • 特記事項

    Mouse species is recommended by WB application. We have preliminary internal testing data to indicate this antibody may not react with mouse in IHC.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab178708の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
IP
1/10 - 1/100.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
IP
1/10 - 1/100.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt.

ターゲット情報

  • 組織特異性

    Liver.

  • 関連疾患

    Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1) [MIM:259900]; also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract.

  • 配列類似性

    Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

  • 細胞内局在

    Peroxisome. Mitochondrion matrix. Except in some HP1 patients where AGT is found in the mitochondrial matrix.
  • Information by UniProt

  • 参照データベース

    • 別名

      • AGT antibody
      • AGT1 antibody
      • Agxt antibody
      • AGXT1 antibody
      • Alanine glyoxylate aminotransferase antibody
      • Alanine glyoxylate aminotransferase3 antibody
      • Alanine--glyoxylate aminotransferase antibody
      • EC 2.6.1.44 antibody
      • EC 2.6.1.51 antibody
      • Hepatic peroxisomal alanine glyoxylate aminotransferase antibody
      • Hepatic peroxisomal alanine:glyoxylate aminotransferase antibody
      • L alanine glyoxylate aminotransferase 1 antibody
      • MS773 antibody
      • PH1 antibody
      • Serine pyruvate aminotransferase antibody
      • Serine--pyruvate aminotransferase antibody
      • Serine--pyruvate aminotransferase, mitochondrial antibody
      • Serine:pyruvate aminotransferase antibody
      • SPAT antibody
      • SPT antibody
      • SPYA_HUMAN antibody
      • TLH6 antibody
      see all

    画像

    • Western blot - Anti-AGXT antibody [EPR13232] (ab178708)
      Western blot - Anti-AGXT antibody [EPR13232] (ab178708)
      All lanes : Anti-AGXT antibody [EPR13232] (ab178708) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Human fetal liver tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP-conjugated at 1/2000 dilution

      Predicted band size: 43 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] (ab178708)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] (ab178708)

      Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling AGXT with ab178708 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] (ab178708)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] (ab178708)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AGXT with ab178708 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-AGXT antibody [EPR13232] (ab178708)
      Immunoprecipitation - Anti-AGXT antibody [EPR13232] (ab178708)

      Western blot analysis on immunoprecipitation pellet from (1) Human fetal liver cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab170891 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

    • Western blot - Anti-AGXT antibody [EPR13232] (ab178708)
      Western blot - Anti-AGXT antibody [EPR13232] (ab178708)

      Western blot analysis of Mouse liver (20μg) labelling AGXT with ab178708 at 1/2000 in 5% NFDM /TBST. A Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L), (ab97051) (1/20000) was used as the secondary antibody.

    • Anti-AGXT antibody [EPR13232] (ab178708)
      Anti-AGXT antibody [EPR13232] (ab178708)

    参考文献 (1)

    ab178708 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab178708 は 1 報の論文で使用されています。

    • Just PA  et al. Lkb1 suppresses amino acid-driven gluconeogenesis in the liver. Nat Commun 11:6127 (2020). PubMed: 33257663

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab178708.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com