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リコンビナントRabMAb

Anti-AGPS 抗体 [EPR13120] - N-terminal (ab184186)

Reviews (2) Submit a question
Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
  • Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
  • Flow Cytometry (Intracellular) - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
  • Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
  • Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13120] to AGPS - N-terminal
  • Suitable for: WB, Flow Cyt (Intra), IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-AGPS antibody [EPR13120] - N-terminal
    AGPS 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13120] to AGPS - N-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, Flow Cyt (Intra), IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa, SW480, K562, HepG2 whole cell lysate (ab7900); Human cervical carcinoma tissue; HeLa cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR13120

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184186の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/10000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • パスウェイ

    Glycerolipid metabolism; ether lipid biosynthesis.

  • 関連疾患

    Defects in AGPS are the cause of rhizomelic chondrodysplasia punctata type 3 (RCDP3) [MIM:600121]. RCDP3 is characterized by rhizomelic shortening of femur and humerus, vertebral disorders, cataract, cutaneous lesions and severe mental retardation.

  • 配列類似性

    Belongs to the FAD-binding oxidoreductase/transferase type 4 family.
    Contains 1 FAD-binding PCMH-type domain.

  • 細胞内局在

    Peroxisome membrane. Localized to the inner aspect of the peroxisomal membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AAG5 antibody
    • ADAP-S antibody
    • ADAS antibody
    • ADAS_HUMAN antibody
    • ADHAPS antibody
    • ADPS antibody
    • Aging associated gene 5 protein antibody
    • Aging-associated gene 5 protein antibody
    • aging-associated protein 5 antibody
    • AGPS antibody
    • ALDHPSY antibody
    • Alkyl-DHAP synthase antibody
    • Alkyldihydroxyacetonephosphate synthase antibody
    • Alkyldihydroxyacetonephosphate synthase, peroxisomal antibody
    • Alkylglycerone phosphate synthase antibody
    • Alkylglycerone-phosphate synthase antibody
    • peroxisomal antibody
    • RCDP3 antibody
    see all

画像

  • Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
    Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
    All lanes : Anti-AGPS antibody [EPR13120] - N-terminal (ab184186) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SW480 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 73 kDa

  • Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
    Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
    All lanes : Anti-AGPS antibody [EPR13120] - N-terminal (ab184186) at 1/2000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 73 kDa

  • Flow Cytometry (Intracellular) - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
    Flow Cytometry (Intracellular) - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)

    Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling AGPS with ab184186 at 1/10 dilution (red), compared to a rabbit IgG negative control (green). 

     

     

  • Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
    Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling AGPS with ab184186 at a 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 diluiton. 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)

    Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling AGPS with ab184186 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
    Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)

参考文献 (0)

ab184186 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184186 は論文での使用が確認できていません。

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1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-AGPS antibody [EPR13120] - N-terminal

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.3% Triton X-100
Specification
HeLa
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Elisabeth Storck Saha

Verified customer

投稿 Jun 13 2019

Western blot abreview for Anti-AGPS antibody [EPR13120] - N-terminal

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel)
Loading amount
20 µg
Treatment
siRNA non-targeting (Lane 1) or AGPS (Lane 2), 72h
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 27 2019

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