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リコンビナントRabMAb

Anti-Ago1 抗体 [EPR25441-8] (BSA and Azide free) (ab300153)

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Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)
  • Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)
  • Western blot - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)
  • Western blot - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)
  • Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25441-8] to Ago1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free)
    Ago1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25441-8] to Ago1 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: ChIP,Flow Cyt (Intra),ICC/IF or IHC-P

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate, HeLa transfected with siRNA1 specifically targeting AGO1, whole cell lysate, HeLa transfected with siRNA2 specifically targeting AGO1, whole cell lysate, Rat heart tissue lysate. IP: Mouse lung tissue lysate, HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate.

  • 特記事項

    ab300153 is a carrier free version of ab300152. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300153の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IP
Use at an assay dependent concentration.
追加情報
Is unsuitable for ChIP,Flow Cyt (Intra),ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).

  • 配列類似性

    Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.

  • 細胞内局在

    Cytoplasm > P-body.
  • Information by UniProt

  • 参照データベース

    • 別名

      • AGO1 antibody
      • AGO1_HUMAN antibody
      • Argonaute 1 antibody
      • Argonaute 1 RISC catalytic component antibody
      • Argonaute1 antibody
      • eIF 2C 1 antibody
      • eIF-2C 1 antibody
      • eIF2C 1 antibody
      • EIF2C antibody
      • Eif2c1 antibody
      • Eukaryotic translation initiation factor 2C 1 antibody
      • GERP95 antibody
      • Golgi Endoplasmic Reticulum protein 95 kDa antibody
      • hAgo1 antibody
      • Protein argonaute 1 antibody
      • Protein argonaute-1 antibody
      • Putative RNA binding protein Q99 antibody
      • Putative RNA-binding protein Q99 antibody
      • Q99 antibody
      see all

    画像

    • Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)
      Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)

      Ago1 was immunoprecipitated from mouse lung tissue lysate 10 μg with ab300152 at 1/30 dilution. ab131366 was used as a secondary antibody at 1/5000 dilution.

      Lane 1(Input)
      Mouse lung tissue lysate 10 μg
      Lane 2(+)
      Mouse lung tissue lysate 
      Lane 3(-)
      Rabbit monoclonal IgG (ab172730) instead of ab300152 in mouse lung tissue lysate 

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)
      Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)

      This data was developed using ab300152, the same antibody clone in a different buffer formulation. 

      Ago1 was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with ab300152 at 1/30 dilution. ab131366 was used as a secondary antibody at 1/5000 dilution.

      Lane 1(Input)
      HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg
      Lane 2(+)
      HeLa whole cell lysate
      Lane 3(-)
      Rabbit monoclonal IgG (ab172730) instead of ab300152 in HeLa whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes. 

    • Western blot - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)
      Western blot - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)
      All lanes : Anti-Ago1 antibody [EPR25441-8] (ab300152) at 1/1000 dilution

      Lane 1 : Human lung tissue lysate
      Lane 2 : Mouse lung tissue lysate
      Lane 3 : Mouse heart tissue lysate
      Lane 4 : Rat heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 97 kDa
      Observed band size: 97 kDa



      This data was developed using ab300152, the same antibody clone in a different buffer formulation. 

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      Exposure time: 

      Lane 1: 3 minutes
      Lane 2: 92 seconds
      Lane 3: 15 seconds
      Lane 4: 37 seconds

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29487329).

      The identity of the lower MW band at approximately 37kDa is unknown.

    • Western blot - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)
      Western blot - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)
      All lanes : Anti-Ago1 antibody [EPR25441-8] (ab300152) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HeLa transfected with siRNA1 specifically targeting AGO1, whole cell lysate
      Lane 3 : HeLa transfected with siRNA2 specifically targeting AGO1, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 97 kDa
      Observed band size: 97 kDa


      Exposure time: 3 minutes


      This data was developed using ab300152, the same antibody clone in a different buffer formulation. 

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29487329).

    • Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)
      Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300153 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300153 は論文での使用が確認できていません。

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