Anti-Ago1 抗体 [EPR25441-8] (ab300152)
![Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (AB300152)](https://www.abcam.co.jp/ps/products/300/ab300152/Images/AB300152-485570-ab300152IP2.jpg "Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (AB300152)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25441-8] to Ago1
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Ago1 antibody [EPR25441-8]
Ago1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR25441-8] to Ago1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: ChIP,Flow Cyt (Intra),ICC/IF or IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate, HeLa transfected with siRNA1 specifically targeting AGO1, whole cell lysate, HeLa transfected with siRNA2 specifically targeting AGO1, whole cell lysate, Rat heart tissue lysate. IP: Mouse lung tissue lysate, HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25441-8 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300152の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
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| IP |
1/30.
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| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa). |
|
IP
1/30. |
ターゲット情報
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機能
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). -
配列類似性
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
細胞内局在
Cytoplasm > P-body. - Information by UniProt
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参照データベース
- Entrez Gene: 26523 Human
- Entrez Gene: 236511 Mouse
- Entrez Gene: 313594 Rat
- Omim: 606228 Human
- SwissProt: Q9UL18 Human
- SwissProt: Q8CJG1 Mouse
- Unigene: 22867 Human
- Unigene: 30800 Mouse
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別名
- AGO1 antibody
- AGO1_HUMAN antibody
- Argonaute 1 antibody
see all
画像
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Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (AB300152)
Ago1 was immunoprecipitated from mouse lung tissue lysate 10 μg with ab300152 at 1/30 dilution. ab131366 was used as a secondary antibody at 1/5000 dilution.
Lane 1(Input)
Mouse lung tissue lysate 10 μg
Lane 2(+)
Mouse lung tissue lysate
Lane 3(-)
Rabbit monoclonal IgG (ab172730) instead of ab300152 in mouse lung tissue lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (AB300152)](https://www.abcam.co.jp/ps/products/300/ab300152/Images/AB300152-485568-ab300152IP1.jpg)
Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (AB300152)Ago1 was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with ab300152 at 1.30 dilution. ab131366 was used as a secondary antibody at 1/5000 dilution.
Lane 1(Input)
HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg
Lane 2(+)
HeLa whole cell lysate
Lane 3(-)
Rabbit monoclonal IgG (ab172730) instead of ab300152 in HeLa whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Western blot - Anti-Ago1 antibody [EPR25441-8] (AB300152)](https://www.abcam.co.jp/ps/products/300/ab300152/Images/AB300152-485567-ab300152WB2.jpg)
Western blot - Anti-Ago1 antibody [EPR25441-8] (AB300152)All lanes : Anti-Ago1 antibody [EPR25441-8] (ab300152) at 1/1000 dilution
Lane 1 : Human lung tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Mouse heart tissue lysate
Lane 4 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 97 kDa
Observed band size: 97 kDaBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time:
Lane 1: 3 minutes
Lane 2: 92 seconds
Lane 3: 15 seconds
Lane 4: 37 secondsThe expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29487329).
The identity of the lower MW band at approximately 37kDa is unknown. -
![Western blot - Anti-Ago1 antibody [EPR25441-8] (AB300152)](https://www.abcam.co.jp/ps/products/300/ab300152/Images/AB300152-485564-ab300152WB1.jpg)
Western blot - Anti-Ago1 antibody [EPR25441-8] (AB300152)All lanes : Anti-Ago1 antibody [EPR25441-8] (ab300152) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa transfected with siRNA1 specifically targeting AGO1, whole cell lysate
Lane 3 : HeLa transfected with siRNA2 specifically targeting AGO1, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 97 kDa
Observed band size: 97 kDa
Exposure time: 3 minutesBlocking and dilution buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29487329).
-
![Anti-Ago1 antibody [EPR25441-8] (AB300152)](https://www.abcam.co.jp/ps/products/300/ab300152/Images/AB300152-485563-Benefits-of-Recombinant-antibodies.jpg)
Anti-Ago1 antibody [EPR25441-8] (AB300152)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab300152 は 1 報の論文で使用されています。
- Fossat N et al. Identification of the viral and cellular microRNA interactomes during SARS-CoV-2 infection. Cell Rep 42:112282 (2023). PubMed: 36961814