Anti-Ago1 抗体 (ab5070)
Key features and details
- Rabbit polyclonal to Ago1
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Drosophila melanogaster
- Isotype: IgG
製品の概要
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製品名
Anti-Ago1 antibody
Ago1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ago1 -
由来種
Rabbit -
アプリケーション
適用あり: IP, IHC-P, WBmore details -
種交差性
交差種: Mouse, Drosophila melanogaster -
免疫原
Synthetic peptide corresponding to Drosophila melanogaster Ago1 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab23538) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5070の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | (2) |
Use at an assay dependent concentration. PubMed: 18463631
|
| IHC-P |
Use at an assay dependent concentration. PubMed: 18528333
|
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| WB | (3) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa.
|
| 特記事項 |
|---|
|
IP
Use at an assay dependent concentration. PubMed: 18463631 |
|
IHC-P
Use at an assay dependent concentration. PubMed: 18528333 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. |
ターゲット情報
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機能
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). -
配列類似性
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
細胞内局在
Cytoplasm > P-body. - Information by UniProt
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参照データベース
- Entrez Gene: 36544 Drosophila melanogaster
- Entrez Gene: 236511 Mouse
- SwissProt: Q8CJG1 Mouse
- Unigene: 30800 Mouse
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別名
- AGO1 antibody
- AGO1_HUMAN antibody
- Argonaute 1 antibody
see all
画像
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Western blot - Anti-Ago1 antibody (ab5070)Anti-Ago1 antibody (ab5070) at 1 µg/ml + Drosophila Melanogaster embryo nuclear extract (from melanogaster embryos 0-12Hr) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Western blot - Anti-Ago1 antibody (ab5070)This image is courtesy of an anonymous AbreviewAnti-Ago1 antibody (ab5070) + Drosophila Melanogaster (Fruit Fly) Tissue Lysate at 15 µg
Secondary
Sheep anti-rabbit HRP conjugated IgG Polyclonal
Performed under reducing conditions.
Predicted band size: 97 kDaBlot was blocked with 5% Milk for 1 hour at room temperature.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (49)
ab5070 は 49 報の論文で使用されています。
- Lim LX et al. The Tudor Domain-Containing Protein, Kotsubu (CG9925), Localizes to the Nuage and Functions in piRNA Biogenesis in D. melanogaster. Front Mol Biosci 9:818302 (2022). PubMed: 35425810
- Liu P et al. Exosomes Derived From Hypoxia-Conditioned Stem Cells of Human Deciduous Exfoliated Teeth Enhance Angiogenesis via the Transfer of let-7f-5p and miR-210-3p. Front Cell Dev Biol 10:879877 (2022). PubMed: 35557954
- Gao Y et al. Optimized protocols for RNA-induced silencing complex assembly and cleavage in cultured Drosophila cells. STAR Protoc 3:101596 (2022). PubMed: 35928005
- Rozen-Gagnon K et al. A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines. Sci Rep 11:736 (2021). PubMed: 33436886
- Kingston ER & Bartel DP Ago2 protects Drosophila siRNAs and microRNAs from target-directed degradation, even in the absence of 2'-O-methylation. RNA 27:710-724 (2021). PubMed: 33853897