AB232628

Anti-Aggrecan 抗体 [EPR14664] - BSA and Azide free

Anti-Aggrecan antibody [EPR14664] - BSA and Azide free

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal Aggrecan antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications.

別名を表示する

AGC1, CSPG1, MSK16, ACAN, Aggrecan core protein, Cartilage-specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, Chondroitin sulfate proteoglycan 1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (AB232628)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (AB232628)

Immunohistochemical analysis of paraffin embedded Human cartilage tissue sections labeling Aggrecan using ab186414 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186414).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (AB232628)

ab186414 staining Aggrecan in normal Human placenta tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin embedded section). ab186414 shows negative staining in normal Human placenta tissue section. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186414).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (AB232628)

ab186414 staining Aggrecan in Human colon carcinoma tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin embedded section). ab186414 shows negative staining in Human colon carcinoma tissue section. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186414).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (AB232628)

ab186414 staining Aggrecan in normal Human colon tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin embedded section). ab186414 shows negative staining in normal Human colon tissue section. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186414).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14664

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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製品の詳細

ab232628 is the carrier-free version of ab186414.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Aggrecan also known as aggrecan core protein or chondroitin sulfate proteoglycan 1 is a large proteoglycan vital for cartilage structure and function. It has a mass of approximately 250 kDa. Aggrecan is expressed mostly in cartilage regions such as articular cartilage but it might also be found in other connective tissues. The structure of aggrecan consists of a core protein that attaches to glycosaminoglycan chains allowing it to create hydrated gel structures essential for the resilience and load-bearing properties of cartilage.

Biological function summary

Aggrecan plays a significant role in maintaining the structural integrity of cartilage by providing resistance to compression. This proteoglycan is a central component of the extracellular matrix forming large aggregates with hyaluronic acid and link proteins. These aggregates contribute to the cartilage's osmotic properties helping resist compression during joint movement. Aggrecan's structural properties make it necessary for normal joint function and health.

Pathways

Aggrecan interacts within the cartilage development and maintenance frameworks. Specifically it participates in the regulation of the ECM-receptor interaction pathway and the proteoglycans in cancer pathway. Aggrecan functions alongside proteins such as type II collagen to ensure biomechanical properties of the cartilage. Additionally its enzymatic breakdown by aggrecanases like ADAMTS can impact these pathways leading to various physiological effects.

Aggrecan is closely associated with osteoarthritis and intervertebral disc degeneration. In osteoarthritis the loss of aggrecan through enzymatic degradation leads to reduced joint function and increased pain. The breakdown involves aggrecanases and other matrix metalloproteinases resulting in cartilage degradation. Diseases such as intervertebral disc degeneration also link to the reduced presence or altered structure of aggrecan impacting spine health and mobility.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

See full target information ACAN

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 5:572-578 PubMed37037945

2023

A proteo-transcriptomic map of non-alcoholic fatty liver disease signatures.

Applications

Unspecified application

Species

Unspecified reactive species

Olivier Govaere,Megan Hasoon,Leigh Alexander,Simon Cockell,Dina Tiniakos,Mattias Ekstedt,Jörn M Schattenberg,Jerome Boursier,Elisabetta Bugianesi,Vlad Ratziu,Ann K Daly,Quentin M Anstee

NPJ Regenerative medicine 7:53 PubMed36114225

2022

Chondrogenic primed extracellular vesicles activate miR-455/SOX11/FOXO axis for cartilage regeneration and osteoarthritis treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Sun,Jie Zhao,Qiang Wu,Yuxin Zhang,Yongqing You,Wenbo Jiang,Kerong Dai

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aggrecan 抗体 [EPR14664] - BSA and Azide free

Anti-Aggrecan antibody [EPR14664] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (AB232628)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (AB232628)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (AB232628)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (AB232628)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

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Abcam product promise