Anti-Aggrecan 抗体 [EPR14664] (ab186414)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)](https://www.abcam.co.jp/ps/products/186/ab186414/Images/ab186414-220800-anti-aggrecan-antibody-epr14664-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14664] to Aggrecan
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Aggrecan antibody [EPR14664]
Aggrecan 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14664] to Aggrecan -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human cartilage tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR14664 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186414の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS. -
細胞内局在
Secreted; extracellular matrix. -
参照データベース
- Entrez Gene: 176 Human
- Omim: 155760 Human
- SwissProt: P16112 Human
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別名
- ACAN antibody
- AGC 1 antibody
- AGC1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)
Immunohistochemical analysis of paraffin embedded Human cartilage tissue sections labeling Aggrecan using ab186414 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)](https://www.abcam.co.jp/ps/products/186/ab186414/Images/ab186414-283882-anti-aggrecan-antibody-epr14664-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)Ab186414 staining Aggrecan in normal Human colon tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin embedded section). Ab186414 shows negative staining in normal Human colon tissue section. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)](https://www.abcam.co.jp/ps/products/186/ab186414/Images/ab186414-283884-anti-aggrecan-antibody-epr14664-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)Ab186414 staining Aggrecan in normal Human placenta tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin embedded section). Ab186414 shows negative staining in normal Human placenta tissue section. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)](https://www.abcam.co.jp/ps/products/186/ab186414/Images/ab186414-283883-anti-aggrecan-antibody-epr14664-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)Ab186414 staining Aggrecan in Human colon carcinoma tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin embedded section). Ab186414 shows negative staining in Human colon carcinoma tissue section. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Aggrecan antibody [EPR14664] (ab186414)](https://www.abcam.co.jp/ps/products/186/ab186414/Images/ab186414-5-benefits-of-recombinant-antibodies.png)
Anti-Aggrecan antibody [EPR14664] (ab186414)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (12)
ab186414 は 12 報の論文で使用されています。
- Wang Y et al. Low-dose celecoxib-loaded PCL fibers reverse intervertebral disc degeneration by up-regulating CHSY3 expression. J Nanobiotechnology 21:76 (2023). PubMed: 36864461
- Kamatani T et al. Human iPS cell-derived cartilaginous tissue spatially and functionally replaces nucleus pulposus. Biomaterials 284:121491 (2022). PubMed: 35395453
- Yu H et al. Integrated single-cell analyses decode the developmental landscape of the human fetal spine. iScience 25:104679 (2022). PubMed: 35832888
- Li G et al. Rejuvenation of Senescent Bone Marrow Mesenchymal Stromal Cells by Pulsed Triboelectric Stimulation. Adv Sci (Weinh) 8:e2100964 (2021). PubMed: 34258884
- Luo Z et al. DNMT3B decreases extracellular matrix degradation and alleviates intervertebral disc degeneration through TRPA1 methylation to inhibit the COX2/YAP axis. Aging (Albany NY) 13:20258-20276 (2021). PubMed: 34428744