AB186414

Anti-Aggrecan 抗体 [EPR14664]

Name: Anti-Aggrecan 抗体 [EPR14664] (ab186414) | Abcam
Brand: Abcam Limited
SKU: AB186414
Price: 27000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-Aggrecan antibody [EPR14664]

RabMAb
Recombinant
20ul selling size
詳細を見る

(1 Review)

(18 Publications)

Rabbit Recombinant Monoclonal Aggrecan antibody. Suitable for IHC-P and reacts with Human samples. Cited in 18 publications.

別名を表示する

AGC1, CSPG1, MSK16, ACAN, Aggrecan core protein, Cartilage-specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, Chondroitin sulfate proteoglycan 1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (AB186414)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (AB186414)

Immunohistochemical analysis of paraffin embedded Human cartilage tissue sections labeling Aggrecan using ab186414 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (AB186414)

ab186414 staining Aggrecan in normal Human placenta tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin embedded section). ab186414 shows negative staining in normal Human placenta tissue section. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (AB186414)

ab186414 staining Aggrecan in Human colon carcinoma tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin embedded section). ab186414 shows negative staining in Human colon carcinoma tissue section. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (AB186414)

ab186414 staining Aggrecan in normal Human colon tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin embedded section). ab186414 shows negative staining in normal Human colon tissue section. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14664

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

style

left-column

製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Aggrecan also known as aggrecan core protein or chondroitin sulfate proteoglycan 1 is a large proteoglycan vital for cartilage structure and function. It has a mass of approximately 250 kDa. Aggrecan is expressed mostly in cartilage regions such as articular cartilage but it might also be found in other connective tissues. The structure of aggrecan consists of a core protein that attaches to glycosaminoglycan chains allowing it to create hydrated gel structures essential for the resilience and load-bearing properties of cartilage.

Biological function summary

Aggrecan plays a significant role in maintaining the structural integrity of cartilage by providing resistance to compression. This proteoglycan is a central component of the extracellular matrix forming large aggregates with hyaluronic acid and link proteins. These aggregates contribute to the cartilage's osmotic properties helping resist compression during joint movement. Aggrecan's structural properties make it necessary for normal joint function and health.

Pathways

Aggrecan interacts within the cartilage development and maintenance frameworks. Specifically it participates in the regulation of the ECM-receptor interaction pathway and the proteoglycans in cancer pathway. Aggrecan functions alongside proteins such as type II collagen to ensure biomechanical properties of the cartilage. Additionally its enzymatic breakdown by aggrecanases like ADAMTS can impact these pathways leading to various physiological effects.

Aggrecan is closely associated with osteoarthritis and intervertebral disc degeneration. In osteoarthritis the loss of aggrecan through enzymatic degradation leads to reduced joint function and increased pain. The breakdown involves aggrecanases and other matrix metalloproteinases resulting in cartilage degradation. Diseases such as intervertebral disc degeneration also link to the reduced presence or altered structure of aggrecan impacting spine health and mobility.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

See full target information ACAN

style

left-column

文献 (18)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 18:5293-5309 PubMed40264591

2025

Gubi Decoction Ameliorates Porous Cartilage Endplate in an Intervertebral Disc Degeneration Model Mouse Through Inhibition of NF-κB Activity and Pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sai Yao,Yanan Li,Hongfeng Ruan,Lianguo Wu,Hanbing Zeng

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2412908 PubMed39804951

2025

Rescuing ACE2-Deficiency-Mediated Nucleus Pulposus Senescence and Intervertebral Disc Degeneration by a Nanotopology-Enhanced RNAi System.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiqiang Sun,Zijian Kang,Chen Yan,Yan Yu,Linhui Han,Yangyang Shi,Liang Chen,Jiangang Shi,Yu Chen,Jingchuan Sun

JCI insight 9: PubMed39405183

2024

Identification of LRP1+CD13+ human periosteal stem cells that require LRP1 for bone repair.

Applications

Unspecified application

Species

Unspecified reactive species

Youngjae Jeong,Lorenzo Deveza,Laura Ortinau,Kevin Lei,John R Dawson,Dongsu Park

Biomaterials science 11:7768-7783 PubMed37870786

2023

Combining biomimetic collagen/hyaluronan hydrogels with discogenic growth factors promotes mesenchymal stroma cell differentiation into Nucleus Pulposus like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Prince David Okoro,Antoine Frayssinet,Stéphanie De Oliveira,Léa Rouquier,Gregor Miklosic,Matteo D'Este,Esther Potier,Christophe Hélary

Journal of nanobiotechnology 21:76 PubMed36864461

2023

Low-dose celecoxib-loaded PCL fibers reverse intervertebral disc degeneration by up-regulating CHSY3 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yunhao Wang,Genjiang Zheng,Xiaoxing Xie,Wei Yu,Jianxi Wang,Fazhi Zang,Chen Yang,Qiangqiang Xiao,Rongcheng Zhang,Leixin Wei,Xiaodong Wu,Lei Liang,Peng Cao,Chen Xu,Jing Li,Bo Hu,Tao Zhang,Jinglei Wu,Huajiang Chen

Nature communications 13:7658 PubMed36496445

2022

Sirt6 attenuates chondrocyte senescence and osteoarthritis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Liang Ji,Hua Jiang,Zhuang Li,Rui Geng,Jun Zheng Hu,Yu Cheng Lin,Jun Lu

iScience 25:104679 PubMed35832888

2022

Integrated single-cell analyses decode the developmental landscape of the human fetal spine.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Yu,Donge Tang,Hongwei Wu,Chunhong Li,Yongping Lu,Fang He,Xiaogang Zhang,Yane Yang,Wei Shi,Wenlong Hu,Zhipeng Zeng,Weier Dai,Minglin Ou,Yong Dai

Biomaterials 284:121491 PubMed35395453

2022

Human iPS cell-derived cartilaginous tissue spatially and functionally replaces nucleus pulposus.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Kamatani,Hiroki Hagizawa,Seido Yarimitsu,Miho Morioka,Saeko Koyamatsu,Michihiko Sugimoto,Joe Kodama,Junko Yamane,Hiroyuki Ishiguro,Shigeyuki Shichino,Kuniya Abe,Wataru Fujibuchi,Hiromichi Fujie,Takashi Kaito,Noriyuki Tsumaki

Frontiers in cell and developmental biology 9:703670 PubMed34650969

2021

Roles and Mechanisms of Irisin in Attenuating Pathological Features of Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangfen Li,Xiaofang Zhu,Hongle Wu,Thomas E Van Dyke,Xiaoyang Xu,Elise F Morgan,Wenyu Fu,Chuanju Liu,Qisheng Tu,Dingming Huang,Jake Chen

Aging 13:20258-20276 PubMed34428744

2021

DNMT3B decreases extracellular matrix degradation and alleviates intervertebral disc degeneration through TRPA1 methylation to inhibit the COX2/YAP axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqiang Luo,Yanchao Ma,Tianning Di,Bing Ma,Hongwei Li,Jiangdong An,Yonggang Wang,Haihong Zhang

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aggrecan 抗体 [EPR14664]

Anti-Aggrecan antibody [EPR14664]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (AB186414)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (AB186414)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (AB186414)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (AB186414)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (18)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise