Anti-Aggrecan 抗体 [6-B-4] (ab3778)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [6-B-4] to Aggrecan
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
製品の概要
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製品名
Anti-Aggrecan antibody [6-B-4]
Aggrecan 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [6-B-4] to Aggrecan -
由来種
Mouse -
特異性
This antibody detects recombinant human Aggrecan by Western Blotting. We have been unable to detect endogenous protein in WB with this antibody. However, there are many citations indicating this antibody is able to detect Aggrecan in endogenous sample lysates.
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アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Recombinant human Aggrecan protein. C-terminal 10 His tagged Human Aggrecan recombinant protein (aa 20 to 675). IHC-P: Human cartilage sections.
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特記事項
This product has switched from a hybridoma to recombinant production method on 08th October 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59.04% PBS -
Concentration information loading...
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精製度
Protein A purified -
一次抗体 備考
This antibody detects aggrecan metabolites (intact or matrix protease-catabolised) in human synovial fluid samples. -
ポリ/モノ
モノクローナル -
クローン名
6-B-4 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3778の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 250 kDa.
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IHC-P | (4) |
Use a concentration of 0.05 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 250 kDa. |
IHC-P
Use a concentration of 0.05 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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関連性
Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS. -
細胞内局在
Secreted; extracellular matrix. -
参照データベース
- Entrez Gene: 176 Human
- Omim: 155760 Human
- SwissProt: P16112 Human
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別名
- ACAN antibody
- AGC 1 antibody
- AGC1 antibody
see all
画像
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Anti-Aggrecan antibody [6-B-4] (ab3778) at 1/1000 dilution + C-terminal 10 His tagged Human Aggrecan recombinant protein (aa 20 to 675) 15ng
Secondary
Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 250 kDaBuffer and Concentration: 2% BSA/TBST
Exposure time: 180 seconds
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ab3778 was shown to react with recombinant human Aggrecan protein in western blot.
Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab3778 at 1µg/ml overnight at 4°C. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye®800CW) preabsorbed (ab216772) at a 1/20000 dilution for 1 hour at RT.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)
Secreted staining of cartilage cells.
Staining performed on a Leica BOND RXTM system using the standard protocol F. The section was pre-treated using enzymatic retrieval, for 15mins. The section was then incubated with ab3778, 0.05µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)
No staining in negative control tissue (human normal liver).
Staining performed on a Leica BOND RXTM system using the standard protocol F. The section was pre-treated using enzymatic retrieval, for 15mins. The section was then incubated with ab3778, 0.05µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (199)
ab3778 は 199 報の論文で使用されています。
- Jiang D et al. microRNA-105-5p protects against chondrocyte injury, extracellular matrix degradation, and osteoarthritis progression by targeting SPARCL1. Histol Histopathol 39:483-496 (2024). PubMed: 37534667
- Liu W et al. Dual-engineered cartilage-targeting extracellular vesicles derived from mesenchymal stem cells enhance osteoarthritis treatment via miR-223/NLRP3/pyroptosis axis: Toward a precision therapy. Bioact Mater 30:169-183 (2023). PubMed: 37593145
- Zhang C et al. HSA-MIR-183-3P TARGETING ATAXIA-TELANGIECTASIA MUTATED PROTEIN REGULATION OF NF-ΚB SIGNALING PATHWAY AFFECTS CELLULAR SENESCENCE CAUSED BY DNA DAMAGE IN LUMBAR DISC DEGENERATION. Acta Endocrinol (Buchar) 19:10-18 (2023). PubMed: 37601718
- Qingxin S et al. Programmable DNA hydrogel provides suitable microenvironment for enhancing autophagy-based therapies in intervertebral disc degeneration treatment. J Nanobiotechnology 21:350 (2023). PubMed: 37759249
- Sun W et al. BMP-7 modified exosomes derived from synovial mesenchymal stem cells attenuate osteoarthritis by M2 polarization of macrophages. Heliyon 9:e19934 (2023). PubMed: 37809369