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Anti-AGE 抗体 (ab23722)

Reviews (6)Q&A (22)References (119)
Immunocytochemistry/ Immunofluorescence - Anti-AGE antibody (ab23722)

    Key features and details

    • Rabbit polyclonal to AGE
    • Suitable for: IHC-Fr, WB, IHC-P, ICC/IF, ELISA
    • Reacts with: Species independent
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-AGE antibody
      AGE 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to AGE

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: IHC-Fr, WB, IHC-P, ICC/IF, ELISAmore details

    • 種交差性

      交差種: Species independent

    • 免疫原

      Full length protein corresponding to Human AGE conjugated to bovine serum albumin. Native protein
      (Peptide available as ab129535)

    • 特記事項


      ab23722 is suitable for the detection of different AGE products in tissues, tissue extracts and body fluids.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab23722の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-Fr (1)
    Use at an assay dependent concentration.
    WB (2)
    Use at an assay dependent concentration.
    IHC-P (3)
    Use at an assay dependent concentration. PubMed: 19223295
    ICC/IF
    Use a concentration of 5 µg/ml.
    ELISA
    Use a concentration of 1 µg/ml.
    特記事項
    IHC-Fr
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration. PubMed: 19223295
    ICC/IF
    Use a concentration of 5 µg/ml.
    ELISA
    Use a concentration of 1 µg/ml.

    ターゲット情報

    • 関連性

      The non enzymatic reaction of reducing carbohydrates with lysine side chains and N terminal amino groups of macromolecules (amino acids, proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The latter products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. In long lived tissue proteins, these chemical modifications accumulate with age and may contribute to the pathophysiology of ageing and long term complications of diabetes, atherosclerosis and renal failure.

    • 細胞内局在

      Cell Membrane and Secreted

    • 別名

      • Advanced glycation end products antibody
      • AGEs antibody

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-AGE antibody (ab23722)
      Immunocytochemistry/ Immunofluorescence - Anti-AGE antibody (ab23722)
      ICC/IF image of ab23722 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23722, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    参考文献 (119)

    ab23722 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab23722 は 119 報の論文で使用されています。

    • López-Grueso MJ  et al. Deficiency of Parkinson's Related Protein DJ-1 Alters Cdk5 Signalling and Induces Neuronal Death by Aberrant Cell Cycle Re-entry. Cell Mol Neurobiol 43:757-769 (2023). PubMed: 35182267
    • Abramov A  et al. Bioprosthetic heart valve structural degeneration associated with metabolic syndrome: Mitigation with polyoxazoline modification. Proc Natl Acad Sci U S A 120:e2219054120 (2023). PubMed: 36574676
    • Senatus L  et al. DIAPH1 mediates progression of atherosclerosis and regulates hepatic lipid metabolism in mice. Commun Biol 6:280 (2023). PubMed: 36932214
    • Quadros KRS  et al. Advanced Glycation End Products and Bone Metabolism in Patients with Chronic Kidney Disease. JBMR Plus 7:e10727 (2023). PubMed: 36936360
    • Pfeffer T  et al. Carnosinase-1 Knock-Out Reduces Kidney Fibrosis in Type-1 Diabetic Mice on High Fat Diet. Antioxidants (Basel) 12:N/A (2023). PubMed: 37372000
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 28 Abreviews or Q&A

    Western blot abreview for Anti-AGE antibody

     Average
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Dermal fibroblasts)
    Loading amount
    1.8 µg
    Specification
    Dermal fibroblasts
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Mark Fowler

    Verified customer

    投稿 Jul 20 2006

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-AGE antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rhesus monkey Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Specification
    kidney
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MISS. Addie Luo

    Verified customer

    投稿 Nov 07 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-AGE antibody

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Antigen Retrival solution EDTA buffer pH9
    Permeabilization
    No
    Specification
    Kidney
    Blocking step
    No blocking step used for 15 minute(s) · Concentration: 0.03 · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jul 10 2017

    Western blot abreview for Anti-AGE antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain: Cortex and Hippocampus in a mix)
    Loading amount
    25 µg
    Specification
    Brain: Cortex and Hippocampus in a mix
    Gel Running Conditions
    Reduced Denaturing (12 % SDS-PAGE gel)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MISS. Josephine Böhme

    Verified customer

    投稿 Apr 09 2013

    Question

    I would like to know wich types of AGEs the ab23722 recognises in a whole mouse heart extract. Does it recognise only BSA-AGE and HSA-AGE or it recognises the other types of AGEs like CML, pentosidine, imidazolone, pyraline, crossline, AGP, etc.?

    Read More
    Answer

    We have not tested the cross reactivity with ab23722 and the other types of AGEs that you mentioned. Only BSA-AGE and HSA-AGE have been tested, and the antibody shoes minimial cross reactivity (< 1%) with BSA and HSA alone.

    Read More

    Question

    I would like use antiAGE antibody to pig tissue.

    Does Anti-AGE antibody (ab23722) work to pig?

    Read More
    Answer

    This antibody is specific to AGE which is not species specific. Therefore, this antibody can be used in all species including pig.

    Read More

    Question

    Does this antibody detect endogenous target or only the immunogens?

    Read More
    Answer

    This antibody will detect endogenous and artificial AGEs. Mainly in certain diseases as diabetes, arteriosclerosis and Modbus Alzheimer AGE has been detected in various tissues (eye, kidney, artery, brain etc.). In principle all proteins can be affected, not only albumins.

    Read More

    Question

    Inquiry: Hi, We had purchased ab23722 (anti-AGE antibody) awhile ago and I have come across some problems. Following a standard glycation protocol, we can detect the sample with a new anti-CML antibody (Ab125145), but not the Anti-AGE antibody. I am curious as to how the AGE modified BSA and HSA were prepared to create the immunogen to produce ab23722? As there are dozens of different AGE modifications possible it is of great interest to our group to know the specific modification detected by the anti-AGE antibody and whether the anti-CML and anti-AGE should be targeting similar structures.

    Read More
    Answer

    The immunogen for ab23722 was prepared as follows:
    Under sterile conditions a mixture of BSA and HSA was incubated with D-Glucose in phosphate buffer pH 7.4 at 37°C for some weeks. Low-molecular weight products and D-Glucose were eliminated by chromatography and dialyses against PBS pH 7.4. This final product was used for immunization of rabbits.

    Read More

    Question



    We have purchased some antibodies (Anti-RAGE: ab37647 and Anti-AGE: ab23722) from Abcam and require some technical support in regards to one of them. We are looking to perform a Western blot using the anti-AGE antibody and noticed on the datasheet that there is some cross-reactivity between the antibody and BSA/HSA <1%. Does this mean that the probablility of cross reactivity is <1% or that there is cross reactivity present if the BSA/HSA concentration is <1%. If there is cross reactivity with BSA would you happen to have any recommendations as to what it would be appropriate to dilute this antibody in? I look forward to your response.

    Read More
    Answer

    Thank you for contacting us. The probability of cross-reactivity with BSA is less than 1%.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question


    Looking for “realistic, human, available in tissues in clinical pathology lab” positive control for ab62623, could not find for him.

    Read More
    Answer

    Thank you for contacting us.

    Regarding ab62623, Stressed tissue such as Alzheimer's affected neurons. or Human brain pre treated with 10ug proteinase K for 40mins at 37oC are suitable positive controls.

    Regarding ab23722, human colon tissue was tested successfully.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    1-10 of 28 Abreviews or Q&A

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