Anti-AF9 抗体 (ab154492)
Key features and details
- Rabbit polyclonal to AF9
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-AF9 antibody -
製品の詳細
Rabbit polyclonal to AF9 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
A recombinant fragment corresponding to a region within amino acids 281-532 of Human AF9 (Uniprot ID P42568).
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ポジティブ･コントロール
- THP1 whole cell lysate. HeLa cells. D54 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154492の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 63 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 63 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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関連疾患
Note=A chromosomal aberration involving MLLT3 is associated with acute leukemias. Translocation t(9;11)(p22;q23) with MLL/HRX. The result is a rogue activator protein. -
配列類似性
Contains 1 YEATS domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4300 Human
- Entrez Gene: 70122 Mouse
- Omim: 159558 Human
- SwissProt: P42568 Human
- SwissProt: A2AM29 Mouse
- Unigene: 591085 Human
- Unigene: 288898 Mouse
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別名
- AF 9 antibody
- AF9_HUMAN antibody
- ALL1 fused gene from chromosome 9 protein antibody
see all
画像
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Western blot - Anti-AF9 antibody (ab154492)Anti-AF9 antibody (ab154492) at 1/1000 dilution + THP1 whole cell lysate at 30 µg
Predicted band size: 63 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-AF9 antibody (ab154492)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling AF9 using ab154492 at 1/500 dilution. Right panel costained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF9 antibody (ab154492)Immunohistochemical analysis of paraffin-embedded D54 xenograft , labeling AF9 using ab154492 at 1/750 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab154492 は 3 報の論文で使用されています。
- Zhai J et al. Primate gastrulation and early organogenesis at single-cell resolution. Nature 612:732-738 (2022). PubMed: 36517595
- Liu B et al. Yeats4 drives ILC lineage commitment via activation of Lmo4 transcription. J Exp Med 216:2653-2668 (2019). PubMed: 31434684
- Kuzmina A et al. Super elongation complex promotes early HIV transcription and its function is modulated by P-TEFb. Transcription 8:133-149 (2017). PubMed: 28340332