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Anti-Aequorin 抗体 (ab9096)

Submit a review Q&A (1)References (7)

Key features and details

  • Rabbit polyclonal to Aequorin
  • Suitable for: ELISA, WB
  • Reacts with: Aequorea victoria
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Aequorin antibody

  • 製品の詳細

    Rabbit polyclonal to Aequorin

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ELISA, WBmore details

  • 種交差性

    交差種: Aequorea victoria

  • 免疫原

    Full length native protein (purified) corresponding to Aequorin.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Constituent: Whole serum
  • Concentration information loading...

  • 精製度

    Whole antiserum

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9096の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
WB
1/1000 - 1/10000.
特記事項
ELISA
Use at an assay dependent concentration.
WB
1/1000 - 1/10000.

ターゲット情報

  • 関連性

    Aequorin, from the jellyfish Aequoria victoria, catalyses the oxidation of coelenterazine, but only in the presence of Ca2+ ions.

  • 参照データベース

    • 別名

      • Aequorin 1 antibody
      • Aequorin 2 antibody
      • Aequorin1 antibody
      • Aequorin2 antibody

    参考文献 (7)

    ab9096 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9096 は 7 報の論文で使用されています。

    • Lee GM  et al. The Ycx1 protein encoded by the yeast YDL206W gene plays a role in calcium and calcineurin signaling. J Biol Chem 299:104647 (2023). PubMed: 36965615
    • Thor K  et al. The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity. Nature 585:569-573 (2020). PubMed: 32846426
    • Chan HY  et al. Expression and reconstitution of the bioluminescent Ca(2+) reporter aequorin in human embryonic stem cells, and exploration of the presence of functional IP3 and ryanodine receptors during the early stages of their differentiation into cardiomyocytes. Sci China Life Sci 59:811-24 (2016). PubMed: 27430888
    • Mehlmer N  et al. A toolset of aequorin expression vectors for in planta studies of subcellular calcium concentrations in Arabidopsis thaliana. J Exp Bot 63:1751-61 (2012). WB . PubMed: 22213817
    • Hamorsky KT  et al. A protein switch sensing system for the quantification of sulfate. Anal Biochem 421:172-80 (2012). PubMed: 22067979
    • Rogers KL  et al. Non-invasive in vivo imaging of calcium signaling in mice. PLoS One 2:e974 (2007). WB . PubMed: 17912353
    • Courchesne WE & Ozturk S Amiodarone induces a caffeine-inhibited, MID1-depedent rise in free cytoplasmic calcium in Saccharomyces cerevisiae. Mol Microbiol 47:223-34 (2003). PubMed: 12492866

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Dear Sir or Madame,
    I am looking for Antibodies against Fluorescent Proteins to be used in Immunohistochemistry. I want to perform tripple-stainings against GFP, dsRed and an additional fluorescent Protein.
    Which additional fluorescent Protein and which of your ABs would you recommend?
    Ideally it can be combined with your Living Color dsred Polyclonal (raised in rabbit, Cat. 632496) and an anti-GFP (raised in chicken) which we are currently using to stain for our constructs containing the dsRed- and GFP-protein.
    Best regards,

    Read More
    Answer

    Thank you for contacting us.

    We are happy to help customers find the most suitable products for their research purposes; however there are many fluorescent proteins and we are not specialized in this particular field. We donot want to recommend a product that may be unsuitable or not the best choice. I would thereforeencourageyou to consult the latest literature available through PubMed and other resources in order to find the most adapted fluorescent protein to complement your immunohistochemistry.

    Improved blue fluorescent proteins are for example described here:
    http://www.ncbi.nlm.nih.gov/pubmed/17444659

    Additional information source may be ˜
    http://www.scholarpedia.org/article/Fluorescent_proteins
    http://www.nature.com/nature/journal/v450/n7166/abs/nature06293.html
    http://www.conncoll.edu/ccacad/zimmer/GFP-ww/GFP-1.htm


    As antibodies, in addition to all the anti GFP/EGFP/YFP/RFP antibodies,we do have an antibody against the blue fluorescent protein ab32791, which is a mouse monoclonal antibody or the anti aequorin antibody, which is a rabbit polyclonal, ab9096.
    https://www.abcam.com/Enhanced-Blue-Fluorescent-Protein-antibody-3A6-ab32791.html (or use the following: https://www.abcam.com/Enhanced-Blue-Fluorescent-Protein-antibody-3A6-ab32791.html).
    https://www.abcam.com/Aequorin-antibody-ab9096.html (or use the following: https://www.abcam.com/Aequorin-antibody-ab9096.html).

    I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again if you would need some more specific information on any of our products.

    I wish you good success withyour research!

    Read More

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