Anti-AE2 抗体 (ab42687)
Key features and details
- Rabbit polyclonal to AE2
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-AE2 antibody
AE2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AE2This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
-
由来種
Rabbit -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Orangutan
-
免疫原
Synthetic peptide corresponding to Human AE2 aa 200-300.
(Peptide available asab42686) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Plasma membrane anion exchange protein of wide distribution. -
配列類似性
Belongs to the anion exchanger (TC 2.A.31) family. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 404084 Cow
- Entrez Gene: 791243 Horse
- Entrez Gene: 6522 Human
- Entrez Gene: 20535 Mouse
- Entrez Gene: 100172030 Orangutan
- Entrez Gene: 100009159 Rabbit
- Entrez Gene: 24780 Rat
- Omim: 109280 Human
see all -
別名
- AE 2 antibody
- AE2 anion exchanger antibody
- Anion exchange protein 2 antibody
see all
画像
-
Immunocytochemistry/ Immunofluorescence - Anti-AE2 antibody (ab42687)ICC/IF image of ab42687 stained MCF7 cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42687, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293 cells fixed in 100% methanol at 5ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (8)
ab42687 は 8 報の論文で使用されています。
- Zhang Z et al. NLRP3 inflammasome-mediated choroid plexus hypersecretion contributes to hydrocephalus after intraventricular hemorrhage via phosphorylated NKCC1 channels. J Neuroinflammation 19:163 (2022). PubMed: 35729645
- Hwang S et al. Intracellular Ca2+-Mediated AE2 Is Involved in the Vectorial Movement of HaCaT Keratinocyte. Int J Mol Sci 21:N/A (2020). PubMed: 33182643
- Hwang S et al. Carbonic anhydrase 12 mutation modulates membrane stability and volume regulation of aquaporin 5. J Enzyme Inhib Med Chem 34:179-188 (2019). PubMed: 30451023
- Hwang S et al. Drug Repurposing as an Antitumor Agent: Disulfiram-Mediated Carbonic Anhydrase 12 and Anion Exchanger 2 Modulation to Inhibit Cancer Cell Migration. Molecules 24:N/A (2019). PubMed: 31546841
- Yin K et al. SLC26A Gene Family Participate in pH Regulation during Enamel Maturation. PLoS One 10:e0144703 (2015). IHC-P ; Rat . PubMed: 26671068
- Loqman MY et al. Suppression of mammalian bone growth by membrane transport inhibitors. J Cell Biochem 114:658-68 (2013). PubMed: 23059814
- Huang J et al. Basolateral chloride loading by the anion exchanger type 2: role in fluid secretion by the human airway epithelial cell line Calu-3. J Physiol 590:5299-316 (2012). PubMed: 22802585
- Kao L et al. Severe Neurologic Impairment in Mice with Targeted Disruption of the Electrogenic Sodium Bicarbonate Cotransporter NBCe2 (Slc4a5 Gene). J Biol Chem 286:32563-74 (2011). IHC-FoFr ; Mouse . PubMed: 21705333